猪骨骼肌卫星细胞分离培养、鉴定及其生物学特性

秦本源,杨阳,张燕伟,刘敏,张万锋,王海珍,吴怡琦,张雪莲,蔡春波,高鹏飞,郭晓红,李步高,曹果清(

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Isolation, Culture, Identification and Biological Characteristics of Pig Skeletal Muscle Satellite Cells

QIN BenYuan,YANG Yang,ZHANG YanWei,LIU Min,ZHANG WanFeng,WANG HaiZhen,WU YiQi,ZHANG XueLian,CAI ChunBo,GAO PengFei,GUO XiaoHong,LI BuGao,CAO GuoQing(

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图1. 骨骼肌卫星细胞形态
A：刚分离的骨骼肌卫星细胞呈球形,折光性强;B：部分卫星细胞开始细胞贴壁,向四周延展;C：分离培养48 h卫星细胞呈梭形或纺锤形;D：分离培养72 h卫星细胞数量增加出现汇合;E：细胞生长至70%时的形态;F：培养5 d细胞拉长变细呈有规律排列;G：培养7 d卫星细胞自发融合,形成多核肌管;H：复苏第3次传代后冻存的骨骼肌卫星细胞

Fig. 1. The morphology of skeletal muscle satellite cells (100×)
A: The separated skeletal muscle satellite cells are spherical and have strong refractive index; B: Some satellite cells begin to adhere and extend to the periphery; C: 48 h of satellite cells are fusiform or spindle-shaped; D: Separated and cultured for 72 h. The number of satellite cells increased and converged; E: Morphology of cells growth to 70%; F:Cells elongated and thinned regularly after cultured 5 days; G: 7 days of satellite cells spontaneously fused to form multinucleated myotubes; H: Recovery skeletal muscle satellite cells frozen after 3 passages