猪骨骼肌卫星细胞分离培养、鉴定及其生物学特性
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秦本源,杨阳,张燕伟,刘敏,张万锋,王海珍,吴怡琦,张雪莲,蔡春波,高鹏飞,郭晓红,李步高,曹果清
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Isolation, Culture, Identification and Biological Characteristics of Pig Skeletal Muscle Satellite Cells
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BenYuan QIN,Yang YANG,YanWei ZHANG,Min LIU,WanFeng ZHANG,HaiZhen WANG,YiQi WU,XueLian ZHANG,ChunBo CAI,PengFei GAO,XiaoHong GUO,BuGao LI,GuoQing CAO
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表1 qRT-PCR引物
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Table 1 Primers for qRT-PCR
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| 基因名称Gene name | 引物序列（5′→3′）Primer sequence | 产物长度Product length (bp) | 序列号Accession No. | | MyoD | F: GAGAGCACTACAGCGGTGAC
R: CCTCGCTGTAATAGGTGCCG | 132 | NM_001002824.1 | | MyoG | F: CTCAGCTCCCTCAACCAGGA
R: GGAGTGCAGATTGTGGGCAT | 195 | NM_001012406.1 | | FABP4 | F:TGGTACAGGTGCAGAAGTGG
R: TTCTGGTAGCCGTGACACCT | 108 | NM_001002817.1 | | CEBP/β | F:CTGGAGACGCAGCATAAGGT
R:TGCTTGAACAAGTTCCGCAG | 110 | NM_001199889.1 | | PPARγ | F:CTATTCCATGCTGTCATGGGTG
R: ACCATGGTCACCTCTTGTGA | 107 | NM_214379.1 | | HSL | F:CACTGACTGCTGACCCCAAG
R:TCCTCACTGTCCTGTCCTTCAC | 121 | NM_214315.3 | | BGLAP | F: GCCACACTCTGCCTTGCTG
R: CTCCTGGAAGCCGATGTGA | 237 | NM_001164004.1 | | RUNX2 | F: GAGTGGACGAGGCAAGAGTT
R:ACTTGGTGCAGAGTTCAGGG | 256 | XM_003482203.4 | | 18S | F:CCCACGGAATCGAGAAAGAG
R: TTGACGGAAGGGCACCA | 122 | NW_018085108.1 |
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