转录因子CsWRKY61对柑橘溃疡病抗性的影响
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龙琴,杜美霞,龙俊宏,何永睿,邹修平,陈善春
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Effect of Transcription Factor CsWRKY61 on Citrus Bacterial Canker Resistance
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Qin LONG,MeiXia DU,JunHong LONG,YongRui HE,XiuPing ZOU,ShanChun CHEN
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表3 转基因植株外源基因拷贝数实时荧光定量PCR分析
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Table 3 qRT-PCR analysis of copy number of transgenes in transgenic lines
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株系
Line | 2-ΔΔCt值2-ΔΔCt value | 预测拷贝数
Estimated copy number | | GUS | NPTII | | W50-1 | 1.32 | 1.39 | 1 | | W50-3 | 1.50 | 1.26 | 1 | | W50-5 | 1.90 | 1.64 | 2 | | W50-9 | 3.56 | 3.72 | 4 | | W50-10 | 2.84 | 2.76 | 3 | | W50-11 | 2.84 | 2.51 | 3 | | W61-1 | 1.07 | 1.47 | 1 | | W61-3 | 1.14 | 1.55 | 1 | | W61-5 | 1.07 | 0.98 | 1 | | W61-6 | 1.33 | 1.63 | 1 | | W61-7 | 1.13 | 1.14 | 1 | | W61-9 | 0.82 | 1.05 | 1 | | W61-11 | 0.97 | 0.82 | 1 | | W61-12 | 1.22 | 1.32 | 1 | | W72-1 | 2.57 | 2.41 | 2 | | W72-2 | 1.62 | 1.90 | 2 | | W72-4 | 2.68 | 2.96 | 3 | | W72-5 | 1.28 | 1.30 | 1 | | W72-7 | 1.32 | 1.49 | 1 | | W72-10 | 1.04 | 1.12 | 1 |
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