转录因子CsWRKY61对柑橘溃疡病抗性的影响

龙琴,杜美霞,龙俊宏,何永睿,邹修平,陈善春

Effect of Transcription Factor CsWRKY61 on Citrus Bacterial Canker Resistance

Qin LONG,MeiXia DU,JunHong LONG,YongRui HE,XiuPing ZOU,ShanChun CHEN

表1 基因克隆和转基因植株鉴定所用引物

Table 1 Primers for gene cloning and identification of transgenic plants

引物名称 Primer name 引物序列 Primer sequence (5′-3′) WRKY50-f ATC GGATCC ATGTCTAATATAAATCTTAT WRKY50-r CTG GTCGAC CTAAGGATTGCTTTGGTGAG WRKY61-f ACC AGATCT ATGGAGGAGAAAAGAGCTAT WRKY61-r TTA GTCGAC TCAGTCAGTGTGCTCTCTAT WRKY72-f TAG GGATCC ATGGAGGTTTTATTGAAAATG WRKY72-r CAT GTCGAC TCAACTGCTTTGATCCTTGT NPTII-f GGATTGCACGCAGGTTCTCCG NPTII-r TCAGAAGAACTCGTCAAGAAGGCG s-f TCTTCGTCAACATGGTGGAGCACGA 50-r ACTCATCAAAGGTCAAGTACTCAG 61-r TCTGGCAGTTTCAAGCTGATCATC 72-r ACAAGTTCAGACTCCATGATTTGAC