烟草PR3b转录后剪切元件NRSE1与GUS融合表达后的可变剪切
赵雪1,王锋2,王文静1,刘晓峰1,卞士权1,刘艳华1,刘新民1,杜咏梅1,张忠锋1,张洪博1(
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Splicing Property Analyses of the NRSE1 Element from Tobacco PR3b mRNA After Fusion Expression with GUS Gene
ZHAO Xue1,WANG Feng2,WANG WenJing1,LIU XiaoFeng1,BIAN ShiQuan1,LIU YanHua1,LIU XinMin1,DU YongMei1,ZHANG ZhongFeng1,ZHANG HongBo1()
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图5. 乙烯（ET）和茉莉酸（JA）处理对转基因烟草的GUS活性影响
A：转基因烟草的GUS活性检测;B：融合表达基因的分子剪切分析,星号为引物二聚体;C：ET、JA处理后转基因植株的GUS基因相对表达量。WT+为转基因野生型烟草,nic1和nic2为转基因低烟碱突变体材料;Mock为未处理材料,ET和JA分别为乙烯和茉莉酸处理材料
Fig. 5. Effects of ET and JA treatment on the GUS activities of transgenic tobacco plants
A: GUS activity of transgenic tobacco; B: Splicing analysis of the fusion of NRSE1 element and GUS. Asterisk indicates primer dimers; C: Relative expression of GUS gene after transgenic plants treated with ET and JA. WT+ indicates transgenic plant of wild type; nic1 and nic2 indicate transgenic plant of low-nicotine mutants. Mock indicates control treatment; ET and JA indicate ethylene and jasmonate treatment, respectively
