烟草PR3b转录后剪切元件NRSE1与GUS融合表达后的可变剪切
赵雪1,王锋2,王文静1,刘晓峰1,卞士权1,刘艳华1,刘新民1,杜咏梅1,张忠锋1,张洪博1(
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Splicing Property Analyses of the NRSE1 Element from Tobacco PR3b mRNA After Fusion Expression with GUS Gene
ZHAO Xue1,WANG Feng2,WANG WenJing1,LIU XiaoFeng1,BIAN ShiQuan1,LIU YanHua1,LIU XinMin1,DU YongMei1,ZHANG ZhongFeng1,ZHANG HongBo1()
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图4. PR3在nic1和nic2突变体转基因材料中的分子剪切
WT+为野生型转基因烟草,nic1和nic2为低烟碱突变体转基因材料,Ctrl为未转基因野生型烟草对照。NRSE1-GUS表示PR3b的NRSE1元件与GUS融合表达。星号为引物二聚体
Fig. 4. Molecular splicing analysis of the fusion of NRSE1 element and GUS in nic1 and nic2 mutants
WT+ indicates transgenic wild type tobacco; nic1 and nic2 indicate the transgenic plants of low-nicotine mutants. Ctrl indicates untransgenic wild type tobacco. PR3b-GUS indicates the fusion of NRSE1 element and GUS gene. Asterisk indicates primer dimers
