烟草PR3b转录后剪切元件NRSE1与GUS融合表达后的可变剪切
赵雪1,王锋2,王文静1,刘晓峰1,卞士权1,刘艳华1,刘新民1,杜咏梅1,张忠锋1,张洪博1(
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Splicing Property Analyses of the NRSE1 Element from Tobacco PR3b mRNA After Fusion Expression with GUS Gene
ZHAO Xue1,WANG Feng2,WANG WenJing1,LIU XiaoFeng1,BIAN ShiQuan1,LIU YanHua1,LIU XinMin1,DU YongMei1,ZHANG ZhongFeng1,ZHANG HongBo1()
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图3. 转基因植株的鉴定
A：转基因植株的RT-PCR分子鉴定;B：转基因材料GUS染色鉴定。Ctrl为非转基因野生型烟草对照;WT+为转基因野生型烟草;nic1和nic2为低烟碱突变体转基因材料。PR3b-GUS表示PR3b的NRSE1元件与GUS融合表达
Fig. 3. Identification of transgenic plants
A: Identification of transgenic plants by RT-PCR; B: Identification of transgenic plants by GUS staining. Ctrl indicates untransgenic wild type tobacco. WT+ indicates transgenic wild type tobacco; nic1 and nic2 indicate the transgenic plants of low-nicotine mutants. PR3b-GUS indicates the fusion of NRSE1 element and GUS gene
