苯磺隆胁迫下甘蓝型油菜萌发期关联性状的QTL定位及候选基因筛选

王刘艳,王瑞莉,叶桑,郜欢欢,雷维,陈柳依,吴家怡,孟丽姣,袁芳,唐章林,李加纳,周清元,崔翠

QTL Mapping and Candidate Genes Screening of Related Traits in Brassica napus L. During the Germination Under Tribenuron-Methyl Stress

LiuYan WANG,RuiLi WANG,Sang YE,HuanHuan GAO,Wei LEI,LiuYi CHEN,JiaYi WU,LiJiao MENG,Fang YUAN,ZhangLin TANG,JiaNa LI,QingYuan ZHOU,Cui CUI

表6 苯磺隆耐性相关性状的候选基因

Table 6 A summary of candidate genes associated with tribenuron-methyl related traits

性状 Trait 位点 Loci 染色体 Chr. 物理区间 Physical interval (bp) 候选基因 Candidate gene 拟南芥基因 Arabidopsis gene 基因 Gene 描述 Description 相对发 芽势 RGV qRGVC07-1 C07 33957781—34814007 BnaC07g29830D AT5G24660 LSU2 细胞氧化剂解毒;对防御反应调控 Cellular oxidant detoxification; Regulation of defense response qRGVA01-1 A01 6196365—6753945 BnaA01g12870D AT4G23030 / MATE家族蛋白 MATE efflux family protein 相对发 芽率 RGR qRGRA01-1 A01 6196365—6753945 BnaA01g12870D AT4G23030 / MATE家族蛋白 MATE efflux family protein qRGRA03-1 A03 14987907—15498700 BnaA03g31340D AT3G10670 ABCI6 参与生物发生或修复氧化破坏的Fe-S簇 Involved in the biogenesis or repair of oxidatively damaged Fe-S clusters. 相对 根长 RRL qRRLA04-1 A04 14446411—15117001 BnaA04g19030D AT2G32740 GT13 半乳糖基转移酶13 Galactosyltransferase 13 BnaA04g17910D AT2G30860 GSTF9 编码GST phi类谷胱甘肽转移酶 Encodes glutathione transferase belonging to the phi class of GSTs BnaA04g18440D AT2G31790 UGT UDP-糖基转移酶超家族蛋白 UDP-Glycosyltransferase superfamily protein qRRLA04-2 A04 15913314—16484458 BnaA04g21090D AT2G36760 UGT73C2 UDP-葡萄糖基转移酶73C2 UDP-glucosyl transferase 73C2 qRRLA04-3 A04 17814614—17923085 BnaA04g24370D AT2G42250 CYP712A1 CYP712A的成员 Member of CYP712A qRRLA06-1 A06 21396095—21528844 BnaA06g31960D AT4G39510 CYP96A12 CYP96A的成员 Member of CYP96A BnaA06g32050D AT4G27710 CYP709B3 CYP709B的成员 Member of CYP709B qRRLA06-2 A06 21528844—22092492 BnaA06g32370D AT3G25180 CYP82G1 编码细胞色素P450单加氧酶 Encodes a cytochrome P450 monooxygenase BnaA06g32970D AT3G26290 CYP71B26 细胞色素P450成员 Putative cytochrome P450 BnaA06g32980D AT2G02580 CYP71B9 CYP71B的成员 Member of CYP71B BnaA06g32990D AT3G26300 CYP71B34 细胞色P450 Putative cytochrome P450 BnaA06g33000D AT3G48560 ALS 催化乙酰乳酸的形成;被磺酰脲类除草剂抑制等 Catalyzing the formation of acetolactate; Inhibited by the sulphonylurea herbicide. etc BnaA06g33010D AT3G26220 CYP71B3 细胞色素P450单加氧酶 Cytochrome P450 monooxygenase BnaA06g33020D AT3G26210 CYP71B23 细胞色素P450成员 Putative cytochrome P450 BnaA06g33030D AT3G26200 CYP71B22 细胞色素P450成员 Putative cytochrome P450 BnaA06g33040D AT3G26190 CYP71B21 细胞色素P450成员 Putative cytochrome P450 BnaA06g33060D AT3G26170 CYP71B19 细胞色素P450成员 Putative cytochrome P450 BnaA06g33050D AT3G26180 CYP71B20 细胞色素P450成员 Putative cytochrome P450 BnaA06g33070D AT3G26165 CYP71B18 细胞色素P450成员 Putative cytochrome P450. qRRLC01-1 C01 3631449—3936101 BnaC01g06930D AT1G13080 CYP71B2 细胞色素P450单加氧酶 Cytochrome P450 monooxygenase 相对 干重 RDW qRDWA02-1 A02 8524524—9460659 BnaA02g15990D AT1G72450 JAZ6 调节防御反应及茉莉酸介导的信号传导途径Regulation of defense response and jasmonic acid mediated signaling pathway BnaA03g22720D AT1G72210 BHLH96 BHLH DNA结合超家族蛋白 BHLH DNA-binding superfamily protein BnaA02g15580D AT2G46960 CYP709B1 CYP709B的成员 Member of CYP709B qRDWA03-2 A03 10805287—12106754 BnaA03g22720D AT2G26480 UGT76D1 UDP-葡萄糖基转移酶76D1 UDP-glucosyl transferase 76D1 BnaA03g25050D AT4G12320 CYP706A6 CYP706A的成员 Member of CYP706A qRDWA04-1 A04 1637957—2461363 BnaA04g03050D AT3G57220 GT 糖基转移酶家族蛋白 Glycosyl transferase family 4 protein qRDWC03-1 C03 47920601—48510329 BnaC03g59160D AT2G25160 CYP82F1 细胞色素P450成员 Cytochrome P450, family 82, subfamily F,