利用CRISPR/Cas9技术编辑水稻香味基因Badh2
祁永斌,张礼霞,王林友,宋建,王建军(
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CRISPR/Cas9 Targeted Editing for the Fragrant Gene Badh2 in Rice
QI YongBin,ZHANG LiXia,WANG LinYou,SONG Jian,WANG JianJun()
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图2. Badh2第2和第7外显子靶点设计及突变类型分析
Start code：起始密码子;Stop code：终止密码子;Exon2：第2外显子;Exon7：第7外显子;sgRNA target site：sgRNA靶点。方框表示靶标序列,红色下划线字体表示PAM序列,蓝色字体表示插入碱基,横线表示缺失碱基,+表示碱基插入,-表示碱基缺失
Fig. 2. Diagram of the sgRNA design and mutation analysis on the exon2 and exon7 of Badh2 gene
The box represented target sequence. The red words with the underline represented PAM sequence. The blue words indicated the insertion base. The transverse line indicated the deletion sequences. + represented base insertion, -indicated base deletion
