利用CRISPR/Cas9技术编辑水稻香味基因Badh2
祁永斌,张礼霞,王林友,宋建,王建军(
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CRISPR/Cas9 Targeted Editing for the Fragrant Gene Badh2 in Rice
QI YongBin,ZHANG LiXia,WANG LinYou,SONG Jian,WANG JianJun()
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图1. CRISPR/Cas9载体示意图
LB：载体左边界;U6：水稻OsU6启动子;SG：向导RNA;UBI：UBI启动子;Cas9：Cas9蛋白;Nos Ter：Nos终止子;35S：35S启动子;Hygro：潮霉素基因;Poly A Ter：PolyA终止子;RB：载体右边界
Fig. 1. Diagram of the map of CRISPR/Cas9
LB: Left border; U6: OsU6 promoter; SG: sgRNA; UBI: UBI promoter; Cas9: Cas9 protein; Nos Ter: Nos terminator; 35S: 35S promoter; Hygro: Hygromycin; Poly A Ter: Poly A terminator; RB: Right border
