利用CRISPR/Cas9技术编辑水稻香味基因Badh2

祁永斌,张礼霞,王林友,宋建,王建军

CRISPR/Cas9 Targeted Editing for the Fragrant Gene Badh2 in Rice

YongBin QI,LiXia ZHANG,LinYou WANG,Jian SONG,JianJun WANG

表1 本研究所用各种引物及序列

Table 1 The primers sequence in this study

引物 Primers 引物序列Primer sequence(5′-3′) 用途Usage SG-SR3 GGCCATTTGTCTGCAGAAT 鉴定载体 Vector verification PUV4-R TCCCAGTCACGACGTTGTAA Cas9-F CATCCAGAAAGCCCAGGTGT 鉴定Cas9 PCR Detection for Cas9 Cas9-R GTTCCTGGTCCACGTACATA Hpt-F GGGTGTCACGTTGCAAGACC 鉴定Hpt PCR Detection for Hpt Hpt-R ATGCCTCCGCTCGAAGTAGC Oligo-up1 TGTGTGGCGATTGCGCGGAGGTACT 构建Exon2靶点二聚体Construct target Oligo for Exon2 Oligo-lw1 AAACAGTACCTCCGCGCAATCGCCA Oligo-up2 TGTGTGATGGCTTCAGCTGCTCCTA 构建Exon7靶点二聚体Construct target Oligo for Exon7 Oligo-lw2 AAACTAGGAGCAGCTGAAGCCATCA exon2-F CACCCTCTGCTTCTGCCTCT Exon2测序 Sequencing for Exon2 exon2-R CAGCCATGCTTCCAACTTATTC exon7-F TGGTCTTCCTTCAGGTGTGC Exon7测序 Sequencing for Exon7 exon7-R TCCAGTGAAACAGGCTGTCA