牛卵泡AGTR2序列结构及表达特性分析

朱芷葳,侯淑宁,郝庆玲,景炅婕,吕丽华,李鹏飞

Sequence Structure and Expression Characteristics Analysis of AGTR2 in Bovine Follicle

ZhiWei ZHU,ShuNing HOU,QingLing HAO,JiongJie JING,LiHua LÜ,PengFei LI

表1 试验中引物序列

Table 1 Primer sequences used in this study

引物名称 Primer name 引物序列 Sequence (5′ to 3′) 退火温度 Tm (℃) 大小 Size (bp) RT-PCR AGTR2-F ATGAAGACCAACTTCTCCCTTGCCAC 58.6 1089 AGTR2-R TTAAGACACAAAGGTCTCCATTTCTCTAAGGG 62.9 qRT-PCR AGTR2-R-F TTCCCTTCCATGTTCTGACC 59.9 96 AGTR2-R-R AAGGAAGTGCCAGGTCAATG 60.1 RPLP0-F CAACCCTGAAGTGCTTGACAT 59.3 115 RPLP0-R AGGCAGATGGATCAGCCA 57.1