农产品中黄曲霉毒素产毒菌标识性分子大容量反应体系 提高ELISA灵敏度

魏晓,张奇,张文,李慧,李培武

Improving the Sensitivity of ELISA by Large-Capacity Reaction System of Aflatoxigenic Fungi-Biomarker in Agro-products

Xiao WEI,Qi ZHANG,Wen ZHANG,Hui LI,PeiWu LI

表1 不同浓度包被抗体和多抗ELISA结果

Table 1 The optimum antibody coating concentration and the best dilution of the polyclonal antibody

包被抗体稀释度 Antibody coating dilution (μg∙mL-1) 多抗稀释度 Polyclonal antibody concentration (μg∙mL-1) 4 3 2.5 2 1.5 1 0.5 0.5 1.4758 0.8651 0.5182 0.3603 0.3344 0.227 0.2148 1 1.5511 0.8667 0.5633 0.4631 0.3676 0.3353 0.2937 2 1.5714 0.9132 0.6063 0.4855 0.3974 0.3598 0.2949 3 1.8485 1.3707 1.0641 0.7815 0.5402 0.3655 0.3570