不同温度下TMV侵染枯斑三生烟的LncRNA差异表达
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贾海燕1,宋丽云1,徐翔1,解屹1,张超群2,刘天波3,赵存孝4,申莉莉1,王杰1,李莹1,王凤龙1,杨金广1
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Differential Expression of LncRNAs in Nicotiana tabacum var. Samsun NN Infected by TMV at Different Temperatures
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HaiYan JIA 1,LiYun SONG 1,Xiang XU 1,Yi XIE 1,ChaoQun ZHANG 2,TianBo LIU 3,CunXiao ZHAO 4,LiLi SHEN 1,Jie WANG 1,Ying LI 1,FengLong WANG 1,JinGuang YANG 1
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图4. 25℃条件下差异表达lncRNA 的qRT-PCR验证 A:25℃和TMV处理下uDElncRNA的相对表达量Relative expression of up-regulated DElncRNAs after TMV treatment at 25℃;B:25℃和TMV处理下dDElncRNA的相对表达量Relative expression of down-regulated DElncRNAs after TMV treatment at 25℃ 误差线表示3组生物学重复的正负标准差,采用t-检验分析差异显著性 Error bars indicate the ±SD of three biological replicates. Student’s t-test. *:P<0.1; **:P<0.01; ***:P<0.001。图5同 The same as Fig. 5
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Fig. 4. Validation of differentially expressed lncRNAs at 25℃ by qRT-PCR
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