马蜂柑响应黄龙病菌不同侵染时期的生物学和转录组学分析

滕彩玲,钟晰,吴昊娣,胡燕,周常勇,王雪峰

Biologic and Transcriptomic Analysis of Citrus hystrix Responses to ‘Candidatus Liberibacter asiaticus’ at Different Infection Stages

CaiLing TENG,Xi ZHONG,HaoDi WU,Yan HU,ChangYong ZHOU,XueFeng WANG

表1 RT-qPCR基因及引物

Table 1 The genes and primers of RT-qPCR

基因标志 Gene symbol 基因ID Gene ID 上游引物序列 Forward primer sequence (5′-3′) 下游引物序列 Reverse primer sequence (5′-3′) 基因功能描述 Description of gene function CESA7 102616000 GGAGTAGGAATGGATGACGAC GGATGCTGGACTTGAGGAAG 纤维素合酶A催化亚基7 Cellulose synthase A catalytic subunit 7 EXPA5 102618439 TCCTGGAGGCTGGTGT CAAGGCGAGCATAAGAC 扩张蛋白A5 Expansin-A5 CalS3 102611841 GGTTGTGATTGCTCTGTGG GTGATTGAAAGCGAGACCTTAG 胼胝质合成酶3 Callose synthase 3 Susy 102616003 GTAGTGGGTGGTGATAGG AACAGTCAAGCCAAAGG 蔗糖合成酶 Sucrose synthase glgC 102615570 TAGGTGGTGGTGCTGG ATTAAACTGGGTGAGGAT 葡萄糖-1-磷酸腺苷转移酶大亚基1 Glucose-1-phosphate adenylyltransferase large subunit 1-like Rboh 102616720 AGGTACTTGCTGGCACA CGTCGGAAATCTGGTC 呼吸爆发氧化酶同源蛋白D Respiratory burst oxidase homolog protein D JAZ 102616825 CCCCACTTCAGTTCCC TGGTATGGTGCTCTTGC 蛋白质TIFY 10A Protein TIFY 10A-like UGT74G1 102613233 TTCGTCATCCTCGTCC CCAACCAGCCCAAACT UDP葡萄糖基转移酶74G1 UDP-glycosyltransferase 74G1 SAMT 102624842 TGAAGCACATAACCCTAG AGAAGACCCACCACCT 水杨酸羧甲基转移酶 Salicylate carboxymethyltransferase PR1 102627194 GACCGTATGGCGAGAA CACCCAATGCGAACC 病程相关蛋白1 Pathogenesis-related protein 1-like R8L3 107176058 TGCGTTGGCTCTGGT AGTCCGTAGTCTTTGTCTCA 抗病性rpp8样蛋白3 Disease resistance RPP8-like protein 3 KTI 102621677 AACTCTTCTTCACCGAATG AGGCTAACTCCACCACC 21 kD种子蛋白21 kD seed protein