转基因油菜筛查阳性质粒分子的研制及应用
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李俊1,李夏莹2,王颢潜2,翟杉杉1,陈子言2,高鸿飞1,李允静1,吴刚1,张秀杰2,武玉花1
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Development and Application of Plasmid Reference Molecule for Genetically Modified Rapeseed Screening
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Jun LI 1,Xia-ying LI 2,Jing-qian WANG 2,Shanshan Zhai 1,Zi-yan CHEN 2,Hong-fei GAO 1,YunJing LI 1,Gang WU 1,Xiu-jie ZHANG 2,Yu-hua WU 1
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图3. 9个筛查元件和2个油菜内标基因的标准曲线绘制和扩增效率检测 E表示扩增效率,R2表示标准曲线的决定系数,slope表示标准曲线的斜率,y-int 表示标准曲线的截距
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Fig. 3. Standard curves and amplification efficiencies of nine screening elements and two rapeseed reference genes E indicates amplification efficiency, R2 indicates regression coefficient, slope indicates the slope of standard curve, y-int indicates the intercept of standard curve
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