利用iTRAQ技术和转录组筛选芍药属远缘杂交不亲和基因

贺丹,谢栋博,张佼蕊,何松林,李朝梅,郑云冰,王政,刘艺平,栗燕,逯久幸

Selected Related Genes about Incompatibility of Distant Hybridization in Paeonia by iTRAQ Analysis and Transcriptome

Dan HE,DongBo XIE,JiaoRui ZHANG,SongLin HE,ChaoMei LI,YunBing ZHENG,Zheng WANG,YiPing LIU,Yan LI,JiuXing LU

表4 不亲和相关的差异基因

Table 4 The differentially expressed genes correlation with cross-incompatibility

基因ID Gene ID KEGG GO注释 GO pathway Swissprot 基因名称 Gene name 表达量 Expression CL268_All K11000/胼胝质酶 Callose enzyme GO:0006075/1,3-β-D-葡聚糖生物合成过程 Biosynthesis of 1,3-β-D-glucan GO:0003843/1,3-β-D-葡聚糖合成酶活性 1,3-β-D-glucan synthetase activity GO:0016021/膜的整体组成部分 Integral part of membrane GO:0000148/1,3-β-D-葡聚糖合成酶复合物 1,3-β-D-glucan synthetase complex 胼胝质合酶5 Callose synthase 5 CalS-5 上调 Up Unigene36776_All 胼胝质合酶12 Callose synthase 12 CalS-12 CL8637_All ko6158/ATP结合盒 ATP-binding cassette GO:0046686/对镉离子的反应 Reaction to cadmium ion GO:0005524/ATP结合 ATP binding GO:0005829/细胞质 Cytoplasm GO:0042742/防御对细菌的反应 Defense against bacterial reactions GO:0016887/ATP酶活性 ATP enzyme activity ABC transporter F family member 3 ABCF 下调 Down CL7449_All ko04075/植物激素信号转导 Phytohormone signal transduction ko01001/蛋白激酶 Protein kinase GO:0046872/结合重金属离子 Binding heavy metal ions GO:0003677/DNA结合核 DNA binding nucleus Squamosa promoter- binding-like protein 16 SPL 上调 Up