利用iTRAQ技术和转录组筛选芍药属远缘杂交不亲和基因
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贺丹,谢栋博,张佼蕊,何松林,李朝梅,郑云冰,王政,刘艺平,栗燕,逯久幸
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Selected Related Genes about Incompatibility of Distant Hybridization in Paeonia by iTRAQ Analysis and Transcriptome
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Dan HE,DongBo XIE,JiaoRui ZHANG,SongLin HE,ChaoMei LI,YunBing ZHENG,Zheng WANG,YiPing LIU,Yan LI,JiuXing LU
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表1 qRT-PCR验证所选基因及对应引物信息
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Table 1 List of the expression profiles selected for confirmation by qRT-PCR
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差异蛋白登记号
DEP Accessions | 基因ID
Gene ID | 引物序列（5′-3′）
Primer sequence (5′-3′) | | gi|327424468_1 | JI445505 | F:TGCTCTCACCTCTCTCCCAAAC | | R:GCCTTGCCTTGACGCTCTTG | | gi|327436891_2 | JI457928 | F:GTGCTTCAAGGTGAGAGAGAGTTC | | R:GCCGAGACAGATAGGATACCATTG | | gi|327427673_4 | JI448710 | F:CGTGTTTGTAGGATTCGTTACTGC | | R:ACGCCATCGGTAGTTGCTTTC | | gi|327426876_5 | JI447913 | F:TTAGCACCAGCACCTTTGAACAG | | R:ACCGTCCGAAGAAGAATGATGATG | | gi|327427197_1 | JI448234 | F:GGACCAAAACGGCTTCATTTCTG | | R:CTCGTCTACTTCCTCATCTGTCAG | | gi|327433480_5 | JI454517 | F:CTTCAGGTCCATAGCCCATCATTG | | R:GTGTCAAGTAATGCTCCGAGTAGG |
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