海南三亚和黄淮海地区玉米小斑病菌致病性及遗传多样性分析

常佳迎,刘树森,石洁,郭宁,张海剑,马红霞,杨春凤

Pathogenicity and Genetic Diversity of Bipolaria maydis in Sanya, Hainan and Huang-Huai-Hai Region

JiaYing CHANG,ShuSen LIU,Jie SHI,Ning GUO,HaiJian ZHANG,HongXia MA,ChunFeng YANG

表2 ISSR引物扩增结果

Table 2 The amplification result with used ISSR primers

引物 Primer 序列 Sequence (5′-3′) 退火温度 Tm (℃) 扩增条带数 Number of amplified bands 多态性条带 Number of polymorphic bands 多态性比率 Polymorphism percent (%) 807 (AG)8T 46 10 8 80.00 808 (AG)8C 46 17 14 82.35 812 (GA)8A 45 7 7 100.00 825 (AC)8T 50 8 5 62.50 834 (AG)8YT 48 14 14 100.00 835 (AG)8YC 50 12 11 91.67 841 (GA)8YC 48 16 14 87.50 856 (AC)8YA 50 8 7 87.50 857 (AC)8YG 51 12 8 66.67 880 (GGAGA)3 47 12 11 91.67 884 HBHA(GA)6G 46 10 8 80.00 885 BHB(GA)7 46 11 8 72.73 887 DVD(TC)7 45 12 6 50.00 888 BDB(CA)7 49 9 4 44.44 889 DBD(AC)7 49 9 8 88.89 891 HVH(TG)7 50 10 7 70.00 总计 Total 177 140 79.10