基于二代测序的甘蓝型油菜白花基因候选区间定位及连锁标记验证

陈雪,王瑞,井付钰,张胜森,贾乐东,段谋正,吴宇

Location and Linkage Markers for Candidate Interval of the White Petal Gene in Brassica napus L. by Next Generation Sequencing

Xue CHEN,Rui WANG,FuYu JING,ShengSen ZHANG,LeDong JIA,MouZheng DUAN,Yu WU

表2 SSR引物序列

Table 2 SSR Primer sequence

引物名 Primer name Chr.C03 SSR 正向引物序列 Forward primer sequence (5′-3′) 反向引物序列 Reverse primer sequence (5′-3′) SSR149 52811241—52811250 (A)10 CCTTAAAGAATACGCTGTGTCT TCGATCACAGAAGCCCCTAAGT SSR154 52859725—52859754 (CTT)10 AGCAAAACCCACATCACTCATT TGGTTGGTTGGTGGAGGAGATA SSR157 52891130—52891141 (CT)6 TGTGGTTCTGGCTGCTGTATTC CCCCACCAACGAGTCCAAAAC SSR161 52941029—52941061 (A)10 GCGGATCAAGCTCTAGTATCT CCAAAGGGGTTAGGTGTTTAGT SSR180 53152240—53152251 (TA)6 TGTGAAGCCCGGTTTGCAACTT ACTCCCACTCTCCCACGATTGT SSR222 53582130—53582142 (A)13 AGAGTCCTTAGCGTTTTGTATT ACGGATGCAGTGGAAGTGAAT