侵染广东连州葫芦的黄瓜绿斑驳花叶病毒的分子特征 及致病性分析
李正刚,农媛,汤亚飞,佘小漫,于琳,蓝国兵,邓铭光,何自福(
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Molecular Characteristic and Pathogenicity Analyses of Cucumber green mottle mosaic virus (CGMMV) Infecting Bottle Gourd in Lianzhou, Guangdong
LI ZhengGang,NONG Yuan,TANG YaFei,SHE XiaoMan,YU Lin,LAN GuoBing,DENG MingGuang,HE ZiFu()
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图3. 利用pCB301-CGMMV侵染性克隆接种本生烟
A：本生烟发病症状Diseased symptoms of N. benthamiana。Mock：未接种对照植株 Control plant without inoculation。下图为白色方框放大图The below figures are magnification of the white frames。B：本生烟接种CGMMV-GDLZ 7 dpi,RT-PCR检测上部叶片发病情况RT-PCR detection of the upper leaves of N. benthamiana at 7 dpi。M：DL2000 DNA Marker。C：Western blot检测上部叶片发病情况。箭头为CGMMV CP所在位置Western blot detection of the upper leaves of N. benthamiana at 7 dpi. The arrow indicates the location of the CGMMV CP。M：Protein marker
Fig. 3. Ago-infiltration into N. benthamiana with pCB301-CGMMV infectious cDNA clones
