玉米pTAC2影响苗期叶片叶绿素合成的转录组分析
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张稳,孟淑君,王琪月,万炯,马拴红,林源,丁冬,汤继华
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Transcriptome Analysis of Maize pTAC2 Effects on Chlorophyll Synthesis in Seedling Leaves
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Wen ZHANG,ShuJun MENG,QiYue WANG,Jiong WAN,ShuanHong MA,Yuan LIN,Dong DING,JiHua TANG
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表1 CRISPR/Cas9系统潜在脱靶位点突变检测
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Table 1 Mutations detected in the putative CRISPR/Cas9 off-target sites
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编号
Serial number | 潜在脱靶位置
Putative off-target locus | 潜在脱靶序列
Sequence of the putative off-target
site (5′-3′) | 错配碱基数
No. of mismatching bases | 测试株数 T0
No. of plants tested | 突变株数 T0
No. of plants with mutations | | Off-target1 | Chr.7:109756398-109756420 | AgCTGGaGTAtAGCCTtATACGG | 4 | 8 | 0 | | Off-target2 | Chr.1 :145160658-145160680 | AcCTGGGGTACAGCCaGATcAGG | 3 | 8 | 0 | | Off-target3 | Chr.6:44283532-44283554 | cGaCCACGCAGCTATGTcTGAGG | 3 | 8 | 0 | | Off-target4 | Chr.4:230687431-230687453 | cGaCCACGCAGCTATGTcTGAGG | 3 | 8 | 0 | | Off-target5 | Chr.7:8936191-8936213 | TGGCCACGCgGCTAcGTgTGGGG | 3 | 8 | 0 | | Off-target6 | Chr.7:8936289-8936311 | TGGCCACGCgGCTAcGTgTGGGG | 3 | 8 | 0 |
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