基于结构类似物的交叉反应性制备高灵敏度 玉米赤霉醇单克隆抗体

胡骁飞,李青梅,姚静静,胡思宇,孙亚宁,邢云瑞,邓瑞广,张改平

Development of High Sensitive Zeranol Monoclonal Antibody Based on the Cross Reactivity of Structural Analogs

XiaoFei HU,QingMei LI,JingJing YAO,SiYu HU,YaNing SUN,YunRui XING,RuiGuang DENG,GaiPing ZHANG

表1 制备的ZAN-O-BSA及ZAN-O-OVA 包板测定ZEN单克隆抗体效价

Table 1 The ZEN McAb’s titer detected by microplates coated with the prepared ZAN-O-BSA or ZAN-O-OVA

ZEN mAb稀释倍数 Dilution ratio of ZEN mAb 1×104 2×104 4×104 8×104 16×104 32×104 64×104 空白 Blank ZAN-O-BSA >3.5 3.044 1.847 1.028 0.579 0.343 0.243 0.064 ZAN-O-OVA 1.182 0.869 0.653 0.387 0.289 0.196 0.163 0.042