梅花鹿激活素A重组蛋白真核表达、纯化 及其生物活性的测定
张宇飞,曹满园,王丽英,赵伟刚,李晓霞,常彤,许保增(
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Eukaryotic Expression, Purification and Biological Activity of Recombinant Cervus Nippon Activin A Protein
ZHANG YuFei,CAO ManYuan,WANG LiYing,ZHAO WeiGang,LI XiaoXia,CHANG Tong,XU BaoZeng()
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图8. Western blot检测激活素A的表达
A：转染空载体pcDNA4/ACTBA的CHO 细胞培养液上清（非还原性SDS-PAGE上样缓存液变性）;B：转染重组质粒pcDNA4/ACTBA的CHO 细胞培养液上清（还原性SDS-PAGE上样缓存液变性）
Fig. 8. Western blot detected the expression of Activin A
A: Culture medium of transfected recombinant plasmid pcDNA4/ACTBA of CHO (SDS-PAGE Loading buffer, non-reducing); B: Culture medium of transfected recombinant plasmid pcDNA4/ACTBA of CHO (SDS-PAGE loading buffer, reducing)
