梅花鹿激活素A重组蛋白真核表达、纯化 及其生物活性的测定
张宇飞,曹满园,王丽英,赵伟刚,李晓霞,常彤,许保增(
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Eukaryotic Expression, Purification and Biological Activity of Recombinant Cervus Nippon Activin A Protein
ZHANG YuFei,CAO ManYuan,WANG LiYing,ZHAO WeiGang,LI XiaoXia,CHANG Tong,XU BaoZeng()
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图7. SDS-PAGE检测激活素A的表达
1：转染空载体pcDNA4/myc-His的CHO 细胞培养液上清;2：转染重组质粒pcDNA4/ACTBA的CHO 细胞培养液上清（非还原性SDS-PAGE上样缓冲液变性）
Fig. 7. SDS-PAGE detected the expression of Activin A
1: Culture medium of transfected empty vector pcDNA4/myc-His of CHO; 2: Culture medium of transfected recombinant plasmid pcDNA4/ACTBA of CHO（SDS-PAGE loading buffer, non-reducing）
