梅花鹿激活素A重组蛋白真核表达、纯化 及其生物活性的测定
张宇飞,曹满园,王丽英,赵伟刚,李晓霞,常彤,许保增(
)
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Eukaryotic Expression, Purification and Biological Activity of Recombinant Cervus Nippon Activin A Protein
ZHANG YuFei,CAO ManYuan,WANG LiYing,ZHAO WeiGang,LI XiaoXia,CHANG Tong,XU BaoZeng()
)
图6. Western blot检测重组质粒在CHO细胞中表达
1：转染重组质粒pcDNA4/ACTBA的CHO 细胞破碎产物;2：转染空载体pcDNA4/myc-His的CHO 细胞破碎产物
Fig. 6. Western blot detected the expression of recombinant plasmid in the cells of CHO
1: Sonication product of transfected recombinant plasmid pcDNA4/ACTBA of CHO; 2: Sonication product of transfected empty vector pcDNA4/myc- His of CHO
