梅花鹿激活素A重组蛋白真核表达、纯化及其生物活性的测定

张宇飞,曹满园,王丽英,赵伟刚,李晓霞,常彤,许保增(

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Eukaryotic Expression, Purification and Biological Activity of Recombinant Cervus Nippon Activin A Protein

ZHANG YuFei,CAO ManYuan,WANG LiYing,ZHAO WeiGang,LI XiaoXia,CHANG Tong,XU BaoZeng(

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图5. ACTBA在CHO细胞中瞬时表达
A1、A2、A3为CHO细胞转染pcDNA4/ACTBA后荧光显微镜照片（A1：DAPI染核,A2：ACTBA-His融合蛋白,A3：重叠后照片）;B1、B2、B3为CHO细胞转染pcDNA4/myc-His空载体后荧光显微镜照片（B1：DAPI染核,B2：阴性对照,B3：重叠后照片）

Fig. 5. Transient expressions of ACTBA gene in CHO cells (×200)
A1,A2,A3: The CHO cells transfected with pcDNA4/ACTBA (A1: Cell stained with DAPI, A2: Expression of ACTBA-His, A3: Merge);B1,B2,B3: The CHO cells transfected with pcDNA4/myc-His (B1: Cell stained with DAPI, B2: Negative control, B3: Merge)