梅花鹿激活素A重组蛋白真核表达、纯化 及其生物活性的测定
张宇飞,曹满园,王丽英,赵伟刚,李晓霞,常彤,许保增(
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Eukaryotic Expression, Purification and Biological Activity of Recombinant Cervus Nippon Activin A Protein
ZHANG YuFei,CAO ManYuan,WANG LiYing,ZHAO WeiGang,LI XiaoXia,CHANG Tong,XU BaoZeng()
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图2. 重组质粒pcDNA4/ACTBA单双酶切鉴定
M1. DNA相对分子质量标准（DL10000）;1. BamHⅠ单酶切pcDNA4/myc-His空质粒;2. BamH I单酶切pcDNA4/ACTBA重组质粒;3. BamH I和Hind III双酶切pcDNA4/ACTBA重组质粒;M2. DNA相对分子质量标准（DL10000）
Fig. 2. Identification of recombinant plasmid pcDNA4/ACTBA by single and double restriction endonuclease digestion
M1. DNA Marker (DL10000); 1. Digested product of pcDNA4/myc-His with BamH I; 2. Digested product of pcDNA4/ACTBA with BamH I; 3. Digested product of pcDNA4/ACTBA with BamH I and Hind III; M2. DNA Marker (DL10000)
