梅花鹿激活素A重组蛋白真核表达、纯化 及其生物活性的测定
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张宇飞,曹满园,王丽英,赵伟刚,李晓霞,常彤,许保增
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Eukaryotic Expression, Purification and Biological Activity of Recombinant Cervus Nippon Activin A Protein
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YuFei ZHANG,ManYuan CAO,LiYing WANG,WeiGang ZHAO,XiaoXia LI,Tong CHANG,BaoZeng XU
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表2 抗体信息表
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Table 2 Antibody information table
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抗体名字
Name of antibody | 生产厂家
Manufacturer | 种属（单抗/多抗）
Species raised (Monoclonal or polyclonal) | 稀释比例
Dilution ratio | | Smad2(L16D3) | Cell Signaling Technology | Mouse (M) | 1:1000 | | p- Smad2(Ser465/467) | Cell Signaling Technology | Rabbit (M) | 1:1000 | | Smad3(C67H9) | Cell Signaling Technology | Rabbit (M) | 1:1000 | | p- Smad3(Ser423/425) | Cell Signaling Technology | Rabbit (M) | 1:1000 | | P450 Aromatase | BIO-RAD | Mouse (M) | 1:200 | | StAR(ab96637) | abcam | Rabbit (P) | 1:1000 | | 6X His tag(ab9108) | abcam | Rabbit(M) | 1:1000 | | β-actin(49900) | abcam | Mouse (M) | 1:10000 | | GAPDH(181602) | abcam | Rabbit (M) | 1:5000 | | Goat Anti-Mouse (HRP) (ab6728) | abcam | Goat (P) | 1:5000 | | Goat Anti-Rabbit (HRP) (ab6721) | abcam | Goat (P) | 1:5000 |
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