梅花鹿激活素A重组蛋白真核表达、纯化 及其生物活性的测定

张宇飞,曹满园,王丽英,赵伟刚,李晓霞,常彤,许保增

Eukaryotic Expression, Purification and Biological Activity of Recombinant Cervus Nippon Activin A Protein

YuFei ZHANG,ManYuan CAO,LiYing WANG,WeiGang ZHAO,XiaoXia LI,Tong CHANG,BaoZeng XU

表1 引物序列与real-time PCR 反应参数

Table 1 Primers sequences and real-time PCR amplification parameters

基因 Gene GenBank登陆号 GenBank accession No. 上下游引物 (5′-3′) Primer 产物长度 Product size (bp) 退火温度 Annealing temperature (℃) Aromatase NM_214427.1 CGCTCAGTCCTGGTCAAAGG ACCAGGAGAGGGGATCTCAC 141 60.0 P450scc NM_214429.1 ACCAGCATGGTGTCTGAAGTT GACCTGGTATTGAAGATGTGTTTT 109 60.0 StAR NM_213755.2 TTAAGCTGTGTGCTGGGAGC CCATGACCCTGAGGTTGGAC 128 60.0 3β-HSD NM_001004049.2 TCGTCCACTTGTTGCTGGAG TGCTCTGGAGCTTAGAAAATTCC 102 60.0 LHR NM_214449.1 ACATAACCACCGTACCAGCA GGAAGGCGTCATTGTGCATC 177 60.0 FSHR NM_214386.3 TCTGGATTTGGGGACCTGGA GAAGGCATCAGGGTCGATGT 150 60.0 GAPDH XM_021091114.1 GTATGATTCCACCCACGGCA CACCCCATTTGATGTTGGCG 122 60.0