梅花鹿激活素A重组蛋白真核表达、纯化 及其生物活性的测定
张宇飞,曹满园,王丽英,赵伟刚,李晓霞,常彤,许保增

Eukaryotic Expression, Purification and Biological Activity of Recombinant Cervus Nippon Activin A Protein
YuFei ZHANG,ManYuan CAO,LiYing WANG,WeiGang ZHAO,XiaoXia LI,Tong CHANG,BaoZeng XU
表1 引物序列与real-time PCR 反应参数
Table 1 Primers sequences and real-time PCR amplification parameters
基因
Gene
GenBank登陆号
GenBank accession No.
上下游引物 (5′-3′)
Primer
产物长度
Product size (bp)
退火温度
Annealing temperature (℃)
Aromatase NM_214427.1 CGCTCAGTCCTGGTCAAAGG
ACCAGGAGAGGGGATCTCAC
141 60.0
P450scc NM_214429.1 ACCAGCATGGTGTCTGAAGTT
GACCTGGTATTGAAGATGTGTTTT
109 60.0
StAR NM_213755.2 TTAAGCTGTGTGCTGGGAGC
CCATGACCCTGAGGTTGGAC
128 60.0
3β-HSD NM_001004049.2 TCGTCCACTTGTTGCTGGAG
TGCTCTGGAGCTTAGAAAATTCC
102 60.0
LHR NM_214449.1 ACATAACCACCGTACCAGCA
GGAAGGCGTCATTGTGCATC
177 60.0
FSHR NM_214386.3 TCTGGATTTGGGGACCTGGA
GAAGGCATCAGGGTCGATGT
150 60.0
GAPDH XM_021091114.1 GTATGATTCCACCCACGGCA
CACCCCATTTGATGTTGGCG
122 60.0