梅花鹿激活素A重组蛋白真核表达、纯化 及其生物活性的测定
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张宇飞,曹满园,王丽英,赵伟刚,李晓霞,常彤,许保增
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Eukaryotic Expression, Purification and Biological Activity of Recombinant Cervus Nippon Activin A Protein
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YuFei ZHANG,ManYuan CAO,LiYing WANG,WeiGang ZHAO,XiaoXia LI,Tong CHANG,BaoZeng XU
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表1 引物序列与real-time PCR 反应参数
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Table 1 Primers sequences and real-time PCR amplification parameters
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基因 Gene | GenBank登陆号 GenBank accession No. | 上下游引物 (5′-3′) Primer | 产物长度 Product size (bp) | 退火温度 Annealing temperature (℃) | Aromatase | NM_214427.1 | CGCTCAGTCCTGGTCAAAGG ACCAGGAGAGGGGATCTCAC | 141 | 60.0 | P450scc | NM_214429.1 | ACCAGCATGGTGTCTGAAGTT GACCTGGTATTGAAGATGTGTTTT | 109 | 60.0 | StAR | NM_213755.2 | TTAAGCTGTGTGCTGGGAGC CCATGACCCTGAGGTTGGAC | 128 | 60.0 | 3β-HSD | NM_001004049.2 | TCGTCCACTTGTTGCTGGAG TGCTCTGGAGCTTAGAAAATTCC | 102 | 60.0 | LHR | NM_214449.1 | ACATAACCACCGTACCAGCA GGAAGGCGTCATTGTGCATC | 177 | 60.0 | FSHR | NM_214386.3 | TCTGGATTTGGGGACCTGGA GAAGGCATCAGGGTCGATGT | 150 | 60.0 | GAPDH | XM_021091114.1 | GTATGATTCCACCCACGGCA CACCCCATTTGATGTTGGCG | 122 | 60.0 |
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