热胁迫对大肠杆菌细胞膜和膜蛋白的影响
张爱静,李琳琼,王鹏杰,高瑀珑

Effects of Heat Stress on Cell Membrane and Membrane Protein of Escherichia coli
AiJing ZHANG,LinQiong LI,PengJie WANG,YuLong GAO
表1 ATCC43889原始对照菌株及其分别经不同温度热胁迫处理并转接培养菌株的细胞膜脂肪酸相对含量
Table 1 Membrane fatty acid composition of ATCC43889 original control strain and the strains which were treated with different temperatures, transfers and incubation, respectively
脂肪酸组成
Fatty acid composition (%)
处理 Treatment
对照菌株 CK 50℃ 60℃ 70℃
C12:0 1.20±0.20c 1.66±0.21c 8.66±1.03a 2.23±0.03b
C13:0 2.08±0.21d 5.20±0.33c 6.67±0.52b 9.88±0.22a
C14:1 11.20±2.03a 10.61±1.36b 10.68±2.15b 8.95±0.78c
C16:0 8.52±0.26c 10.54±0.01b 10.88±0.49b 14.65±1.02a
C16:1 17.09±0.64a 10.22±2.03b 6.65±0.02c 1.78±0.54d
C17:0 8.60±0.87c 8.90±0.25c 9.27±0.33b 13.68±1.27a
C17:1 11.32±0.12a 8.66±0.29b 5.49±0.48d 6.09±0.52c
C18:1n9t 4.99±0.10a 3.23±0.20b 2.33±0.19c 2.85±0.16c
C18:1n9c 8.78±0.68
C18:2n6t 10.02±0.29a 8.06±0.06b 5.87±0.38c 4.93±0.37d
C18:3n3 5.64±0.44
C20:0 1.46±0.02c 8.98±0.23a 6.36±0.98b 9.12±0.10a
C21:0 2.88±0.77
C24:0 6.22±0.04c 9.16±0.11a 8.96±0.02b 9.34±0.03a
SFA 30.96±3.22b 44.44±1.88ab 50.80±1.56a 58.90±2.56a
USFA 69.04±1.35a 40.78±1.47b 31.02±0.07c 24.60±0.30d
S/U 0.45 ±0.01d 1.09±0.15c 1.64±0.06b 2.39±0.10a