热胁迫对大肠杆菌细胞膜和膜蛋白的影响
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张爱静,李琳琼,王鹏杰,高瑀珑
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Effects of Heat Stress on Cell Membrane and Membrane Protein of Escherichia coli
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AiJing ZHANG,LinQiong LI,PengJie WANG,YuLong GAO
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表1 ATCC43889原始对照菌株及其分别经不同温度热胁迫处理并转接培养菌株的细胞膜脂肪酸相对含量
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Table 1 Membrane fatty acid composition of ATCC43889 original control strain and the strains which were treated with different temperatures, transfers and incubation, respectively
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脂肪酸组成
Fatty acid composition (%) | 处理 Treatment | | 对照菌株 CK | 50℃ | 60℃ | 70℃ | | C12:0 | 1.20±0.20c | 1.66±0.21c | 8.66±1.03a | 2.23±0.03b | | C13:0 | 2.08±0.21d | 5.20±0.33c | 6.67±0.52b | 9.88±0.22a | | C14:1 | 11.20±2.03a | 10.61±1.36b | 10.68±2.15b | 8.95±0.78c | | C16:0 | 8.52±0.26c | 10.54±0.01b | 10.88±0.49b | 14.65±1.02a | | C16:1 | 17.09±0.64a | 10.22±2.03b | 6.65±0.02c | 1.78±0.54d | | C17:0 | 8.60±0.87c | 8.90±0.25c | 9.27±0.33b | 13.68±1.27a | | C17:1 | 11.32±0.12a | 8.66±0.29b | 5.49±0.48d | 6.09±0.52c | | C18:1n9t | 4.99±0.10a | 3.23±0.20b | 2.33±0.19c | 2.85±0.16c | | C18:1n9c | 8.78±0.68 | — | — | — | | C18:2n6t | 10.02±0.29a | 8.06±0.06b | 5.87±0.38c | 4.93±0.37d | | C18:3n3 | 5.64±0.44 | — | — | — | | C20:0 | 1.46±0.02c | 8.98±0.23a | 6.36±0.98b | 9.12±0.10a | | C21:0 | 2.88±0.77 | — | — | — | | C24:0 | 6.22±0.04c | 9.16±0.11a | 8.96±0.02b | 9.34±0.03a | | SFA | 30.96±3.22b | 44.44±1.88ab | 50.80±1.56a | 58.90±2.56a | | USFA | 69.04±1.35a | 40.78±1.47b | 31.02±0.07c | 24.60±0.30d | | S/U | 0.45 ±0.01d | 1.09±0.15c | 1.64±0.06b | 2.39±0.10a |
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