用高通量测序技术研究松辽黑猪与长白猪背最长肌 mRNA和lncRNA的差异表达

吴垚群,陈少康,盛熙晖,齐晓龙,王相国,倪和民,郭勇,王楚端,邢凯

Differential Expression of mRNA and lncRNA in Longissimus Dorsi Muscle of Songliao Black Pig and Landrace Pig Based on High-Throughput Sequencing Technique

YaoQun WU,ShaoKang CHEN,XiHui SHENG,XiaoLong QI,XiangGuo WANG,HeMin NI,Yong GUO,ChuDuan WANG,Kai XING

表1 引物序列

Table 1 Primer sequences

基因Gene 上游引物（5′-3′）Forward primer 下游引物（5′-3′）Reverse primer HPSE GCGTTCCTGTCCGTAACCAT ATGCGGGAGACAAACCTCTG BTK TTTCAGCACCATCCCTGAGC ACGGCGCATTCTTGTTTTGT SFT2D2 ACTGTCTTCCTCATGGGACC GTGCAAAACACAACAGCACC SMAD6 CCCATCTTCGTCAACTCCCC GCTCGAAGTCGAACACCTTG FGF10 AGATGTCCGCTGGAGAAAGC TCCAGGATACTGTACGGGCA TECRL CAAAGCATGTGGGCCCTTTT GTGGTCCAACTGACTTGTTGAC PRKD2 CTACAACCGCTCACTGGACAT TGCGTTCTGGATCTGGTCAT TRAPPC2 ACCAGTTCATAGCACACGCT GCCGAGACAAACCACTCATTG HOXD1 CGCACGAATTTCAGCACCAA TCAGCTGCAATGAGTTGGCT ATP2B3 GGAGCAGAAGTTCACCGTCA GGCAGCAGGTCTCCGTATTT TCONS-OOO52690 GGGAAATCCAGCATCGTGTG CCACAAGGCACAGTTTTGGTC TCONS-OOO59635 AGCGTTTATTCCTCCCAGGC AGGTACTTGTAGGGCCGGTA TCONS-OOO35174 CACCGTAGGGTCGTTGCA CATCCTTCCTTCCGTCCAG TCONS-OOO39610 ACACCTTGGGACCCCAACTA GGCCAGAATGACGGATGACA TCONS-OOO59695 CAGGGTTGTCCAGCCAATGT TGCTGGGCTGTCATCCTCTT TCONS-OOO16916 ATTACTTTGCCCCGGAGGTCT TAATTAGGGCTTCCCGGGTCT TCONS-OOO46917 TGCCCGATACTGTAATGCCA CATTCCCCGAGCACGGATTC TCONS-OOO67508 CGTGGACGAAAAACGATCCG CTTCACCTCGTTCAGGGCTT TCONS-OOO36675 GGTCTTCCAAATCGGTTGCC ACAGGGGTTGCTTTGGTGTA TCONS-OOO52912 GGACCTGGACCCAATCAACT TGATGATGGCACGGAATGGT GAPDH TATGATTCCACCCACGGCAA CCATTTGATGTTGGCGGGAT