甜瓜短蔓基因Cmdm1的精细定位及候选基因分析

马建,李丛丛,王建设

Fine Mapping and Candidate Gene Analysis of a Short Internodes Gene Cmdm1 in Melon (Cucumis melo L.)

Jian MA,CongCong LI,JianShe WANG

表1 本研究所用的PCR引物序列

Table 1 PCR primer sequences used in this study

引物名称 Primer 正向引物 Forward primer (5'-3') 反向引物 Reverse primer (5'-3') c7-3 AATTCCCTTACCCTTGACAGCA GTTGAACCGACTCGACCGAC c7-13 CAGGCAGGCTACGCTACATT CGCTCTGACGTTATGAAGTTGT c7-70 GGAGAAAATGACGCTCTTAATTGAT CCCCATCTCCATTACAATGTTTACT c7-94 ATGCATGTTAAGTTGTCCTACGG CCCACCACTATGGCATGTGTAA c7-101 ACATGTCAGAGCAACAGAATTGA AAGCTCCATTCGAGGATTCATT c7-103 ACCTCGTTCATGCCAAAGATG ACCTTTGACTCCAAAAGAAGGA c7-112 GTGTTCGACCAACCAATTTTCAC GGTGTTAGTGTGGATTTGGGA c7-119 AAAACCTTCAAATCATGGACTGTT ACAGTTAAAATAGCATGCAAGAAGT c7-120 AATTTTTAGATCCACCGCTGCT CATACTTCTCCCCAATCTTCCC c7-130 GTGTCATGTCGAAACACCGTC CTTCGTGGTACGTCGGGAAA c7-132 AAAGGTGTAATTCACATTCATCCAA ACTTTGTTGGAACATAGAGTTGGT c7-167 ATTCCACGACTGCCCATCAG TGCTGTCTGCAACATTAAAGGG c7-200 TCGTTTACCGACTACATTGAGC TGTCCAGATTTTAAAGTTGATTCGT c7-206 CAATACATGGCTCTAAAGACAAACT AGAGTGTTTCACTCGCATCCT dm-1 CAACTTCAAAATCTTCTCTCCTTGT TAAACATGCAGCAGGAAGGAAA s2 GGCTGTTGCTTGGACTTTGG AGCATGACCGGCTCATCAAA MELO3C016916 CCAGCCATGAACACTGCAAAA CCATGTTGCTGCTTGCCTAC MELO3C016917 CTCTCCTTGTTGTCACTGTTCAAA ATCTGCTACCCTCAGCTTTCC MELO3C016918 CTTCTGGATCTCGCTAGGGTTT TGGTGTGGCACCTGTGTTT MELO3C016919 TTTGTAGGCTGGCTTTGAGGA ACTCCCCAAAAGAGAAGTCCCA q3C016916 TCGTCCGAACAACACAATACCT CTCATAAACGTGAAGGGCCA CmActin TTCCCTCAGCACTTTCCAACA TCCCTTTCCACCTTTTTGCTTC