烟草Hsc70-2的克隆及对马铃薯Y病毒侵染烟草的促进作用
|
龚明月 1,段啸天 1,余婷婷 1,王杰 2,申莉莉 2,李莹 2,刘明宏 3,李永亮 4,吕洪坤 5,章松柏 1(  ),杨金广 2(  )
|
Cloning of Hsc70-2 and Its Promoting Effect on Potato virus Y Infection in Nicotiana benthamiana
|
MingYue GONG 1,XiaoTian DUAN 1,TingTing YU 1,Jie WANG 2,LiLi SHEN 2,Ying LI 2,MingHong LIU 3,YongLiang LI 4,HongKun LÜ 5,SongBai ZHANG 1(  ),JinGuang YANG 2(  )
|
|
图7. 沉默及过表达NbHsc70-2对PVY积累的影响 A:沉默NbHsc70-2后对PVY积累的影响Effect of silencing NbHsc70-2 on PVY accumulation;B:过表达NbHsc70-2后对PVY积累的影响Effect of overexpression of NbHsc70-2 on PVY accumulation pTRV1与pTRV2::NbHsc70-2或空的pTRV2(作为阴性对照)通过根癌农杆菌共浸润培养3周的本氏烟。侵染7 d后接种PVY开始取样进行检测。pEarleyGate100:: NbHsc70-2或空的pEarleyGate100(作为阴性对照)通过根癌农杆菌共浸润培养3周的本氏烟,提前24 h接种PVY。侵染48 h后开始取样进行检测pTRV1 and pTRV2::NbHsc70-2 or empty pTRV2 (as a negative control) were co-infiltrated with A. tumefaciens for 3 weeks of N. benthamiana. Seven days after infection, PVY was inoculated and samples were taken for test. pEarleyGate100:: NbHsc70-2 or empty pEarleyGate100 (as a negative control) was inoculated with A. tumefaciens for 3 weeks and was inoculated with PVY 24 hours in advance. Sampling was taken 48 h after infection
|
Fig. 7. Effects of NbHsc70-2 silencing and overexpression on PVY accumulation
|
|
 |
|
|