烟草Hsc70-2的克隆及对马铃薯Y病毒侵染烟草的促进作用
龚明月1,段啸天1,余婷婷1,王杰2,申莉莉2,李莹2,刘明宏3,李永亮4,吕洪坤5,章松柏1(),杨金广2()
Cloning of Hsc70-2 and Its Promoting Effect on Potato virus Y Infection in Nicotiana benthamiana
MingYue GONG1,XiaoTian DUAN1,TingTing YU1,Jie WANG2,LiLi SHEN2,Ying LI2,MingHong LIU3,YongLiang LI4,HongKun LÜ5,SongBai ZHANG1(),JinGuang YANG2()

图7. 沉默及过表达NbHsc70-2对PVY积累的影响
A:沉默NbHsc70-2后对PVY积累的影响Effect of silencing NbHsc70-2 on PVY accumulation;B:过表达NbHsc70-2后对PVY积累的影响Effect of overexpression of NbHsc70-2 on PVY accumulation
pTRV1与pTRV2::NbHsc70-2或空的pTRV2(作为阴性对照)通过根癌农杆菌共浸润培养3周的本氏烟。侵染7 d后接种PVY开始取样进行检测。pEarleyGate100:: NbHsc70-2或空的pEarleyGate100(作为阴性对照)通过根癌农杆菌共浸润培养3周的本氏烟,提前24 h接种PVY。侵染48 h后开始取样进行检测pTRV1 and pTRV2::NbHsc70-2 or empty pTRV2 (as a negative control) were co-infiltrated with A. tumefaciens for 3 weeks of N. benthamiana. Seven days after infection, PVY was inoculated and samples were taken for test. pEarleyGate100:: NbHsc70-2 or empty pEarleyGate100 (as a negative control) was inoculated with A. tumefaciens for 3 weeks and was inoculated with PVY 24 hours in advance. Sampling was taken 48 h after infection

Fig. 7. Effects of NbHsc70-2 silencing and overexpression on PVY accumulation