烟草Hsc70-2的克隆及对马铃薯Y病毒侵染烟草的促进作用
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龚明月 1,段啸天 1,余婷婷 1,王杰 2,申莉莉 2,李莹 2,刘明宏 3,李永亮 4,吕洪坤 5,章松柏 1(  ),杨金广 2(  )
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Cloning of Hsc70-2 and Its Promoting Effect on Potato virus Y Infection in Nicotiana benthamiana
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MingYue GONG 1,XiaoTian DUAN 1,TingTing YU 1,Jie WANG 2,LiLi SHEN 2,Ying LI 2,MingHong LIU 3,YongLiang LI 4,HongKun LÜ 5,SongBai ZHANG 1(  ),JinGuang YANG 2(  )
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图6. 沉默NbHsc70-2表型分析 A:沉默NbHsc70-2 7 d后的烟草表型Tobacco phenotype after 7 days of silencing NbHsc70-2;B:沉默NbHsc70-2 7 d后的沉默效率Silencing efficiency after 7 days of silencing NbHsc70-2;C:接种PVY-GFP 7 d后的烟草表型Tobacco phenotype after 7 days of PVY-GFP inoculation;D:接种PVY-GFP 7 d后的烟草相对荧光强度Relative fluorescence intensity of tobacco after 7 days of PVY-GFP inoculation pTRV1与pTRV2::NbHsc70-2或空的pTRV2(作为阴性对照)通过根癌农杆菌共浸润培养3周的本氏烟。侵染7 d后统计NbHsc70-2沉默效率并拍照pTRV1 and pTRV2::NbHsc70-2 or empty pTRV2 (as a negative control) were co-infiltrated with Agrobacterium tumefaciens for 3 weeks of N. benthamiana. The silencing efficiency of NbHsc70-2 was counted 7 days after infection and photos were taken
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Fig. 6. Phenotype analysis of NbHsc70-2 silencing
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