烟草Hsc70-2的克隆及对马铃薯Y病毒侵染烟草的促进作用
龚明月1,段啸天1,余婷婷1,王杰2,申莉莉2,李莹2,刘明宏3,李永亮4,吕洪坤5,章松柏1(
),杨金广2()
),杨金广2()
Cloning of Hsc70-2 and Its Promoting Effect on Potato virus Y Infection in Nicotiana benthamiana
MingYue GONG1,XiaoTian DUAN1,TingTing YU1,Jie WANG2,LiLi SHEN2,Ying LI2,MingHong LIU3,YongLiang LI4,HongKun LÜ5,SongBai ZHANG1(),JinGuang YANG2()
),JinGuang YANG2()
图6. 沉默NbHsc70-2表型分析
A：沉默NbHsc70-2 7 d后的烟草表型Tobacco phenotype after 7 days of silencing NbHsc70-2;B：沉默NbHsc70-2 7 d后的沉默效率Silencing efficiency after 7 days of silencing NbHsc70-2;C：接种PVY-GFP 7 d后的烟草表型Tobacco phenotype after 7 days of PVY-GFP inoculation;D：接种PVY-GFP 7 d后的烟草相对荧光强度Relative fluorescence intensity of tobacco after 7 days of PVY-GFP inoculation
pTRV1与pTRV2::NbHsc70-2或空的pTRV2（作为阴性对照）通过根癌农杆菌共浸润培养3周的本氏烟。侵染7 d后统计NbHsc70-2沉默效率并拍照pTRV1 and pTRV2::NbHsc70-2 or empty pTRV2 (as a negative control) were co-infiltrated with Agrobacterium tumefaciens for 3 weeks of N. benthamiana. The silencing efficiency of NbHsc70-2 was counted 7 days after infection and photos were taken
Fig. 6. Phenotype analysis of NbHsc70-2 silencing
