烟草Hsc70-2的克隆及对马铃薯Y病毒侵染烟草的促进作用

龚明月,段啸天,余婷婷,王杰,申莉莉,李莹,刘明宏,李永亮,吕洪坤,章松柏,杨金广

Cloning of Hsc70-2 and Its Promoting Effect on Potato virus Y Infection in Nicotiana benthamiana

MingYue GONG,XiaoTian DUAN,TingTing YU,Jie WANG,LiLi SHEN,Ying LI,MingHong LIU,YongLiang LI,HongKun LÜ,SongBai ZHANG,JinGuang YANG

表1 试验中采用的引物及序列

Table 1 Sequence of the primers used in this study

引物 Primer 序列 Sequence (5′ to 3′) 限制性内切酶 Restriction enzyme NbHsc70-2 F ATGCGTATTATTAACGAGCCTAC NbHsc70-2 R TTAATCAACTTCCTCAATCTTAGGAC NbHsc70-2-XbaI F CTTTAGATCTTCTAGAATGCGTATTATTAACGAGCCTACTG XbaI NbHsc70-2-KpnI R ATATTAATGTCGACGGTACCATCAACTTCCTCAATCTTAGGACC KpnI RNAi NbHsc70-2 F TAAGGTTACCGAATTCATTCTTTCAGGTGAGGGTAATGAG EcoRI RNAi NbHsc70-2 R AGACGCGTGAGCTCGGTACCCAGGAGGAATACCAGAAAGTTC KpnI NbHsc70-2-AhdI F AGCAGGCTTTGACTTTAGGTCATGCGTATTATTAACGAGCCTACTG AhdI NbHsc70-2-AhdI R TGGGTCTAGAGACTTTAGGTCTTAATCAACTTCCTCAATCTTAGGA AhdI NbHsc70-2qRT-F AGGCTCCACTAGGATTCCGAAGG NbHsc70-2qRT-R AGTGACATCCAACAGCAACAGGTC PVY-CP-F GATGAATGGGCTTATGGTTTGGTG PVY-CP-R GATTTGCCTAAGGGTTGGTTTCG Actin-F CAAGGAAATCACCGCTTTGG Actin-R AAGGGATGCGAGGATGGA