烟草Hsc70-2的克隆及对马铃薯Y病毒侵染烟草的促进作用
龚明月,段啸天,余婷婷,王杰,申莉莉,李莹,刘明宏,李永亮,吕洪坤,章松柏,杨金广

Cloning of Hsc70-2 and Its Promoting Effect on Potato virus Y Infection in Nicotiana benthamiana
MingYue GONG,XiaoTian DUAN,TingTing YU,Jie WANG,LiLi SHEN,Ying LI,MingHong LIU,YongLiang LI,HongKun LÜ,SongBai ZHANG,JinGuang YANG
表1 试验中采用的引物及序列
Table 1 Sequence of the primers used in this study
引物
Primer
序列
Sequence (5′ to 3′)
限制性内切酶
Restriction enzyme
NbHsc70-2 F ATGCGTATTATTAACGAGCCTAC
NbHsc70-2 R TTAATCAACTTCCTCAATCTTAGGAC
NbHsc70-2-XbaI F CTTTAGATCTTCTAGAATGCGTATTATTAACGAGCCTACTG XbaI
NbHsc70-2-KpnI R ATATTAATGTCGACGGTACCATCAACTTCCTCAATCTTAGGACC KpnI
RNAi NbHsc70-2 F TAAGGTTACCGAATTCATTCTTTCAGGTGAGGGTAATGAG EcoRI
RNAi NbHsc70-2 R AGACGCGTGAGCTCGGTACCCAGGAGGAATACCAGAAAGTTC KpnI
NbHsc70-2-AhdI F AGCAGGCTTTGACTTTAGGTCATGCGTATTATTAACGAGCCTACTG AhdI
NbHsc70-2-AhdI R TGGGTCTAGAGACTTTAGGTCTTAATCAACTTCCTCAATCTTAGGA AhdI
NbHsc70-2qRT-F AGGCTCCACTAGGATTCCGAAGG
NbHsc70-2qRT-R AGTGACATCCAACAGCAACAGGTC
PVY-CP-F GATGAATGGGCTTATGGTTTGGTG
PVY-CP-R GATTTGCCTAAGGGTTGGTTTCG
Actin-F CAAGGAAATCACCGCTTTGG
Actin-R AAGGGATGCGAGGATGGA