烟草Hsc70-2的克隆及对马铃薯Y病毒侵染烟草的促进作用
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龚明月,段啸天,余婷婷,王杰,申莉莉,李莹,刘明宏,李永亮,吕洪坤,章松柏,杨金广
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Cloning of Hsc70-2 and Its Promoting Effect on Potato virus Y Infection in Nicotiana benthamiana
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MingYue GONG,XiaoTian DUAN,TingTing YU,Jie WANG,LiLi SHEN,Ying LI,MingHong LIU,YongLiang LI,HongKun LÜ,SongBai ZHANG,JinGuang YANG
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表1 试验中采用的引物及序列
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Table 1 Sequence of the primers used in this study
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引物 Primer | 序列 Sequence (5′ to 3′) | 限制性内切酶 Restriction enzyme | NbHsc70-2 F | ATGCGTATTATTAACGAGCCTAC | | NbHsc70-2 R | TTAATCAACTTCCTCAATCTTAGGAC | | NbHsc70-2-XbaI F | CTTTAGATCTTCTAGAATGCGTATTATTAACGAGCCTACTG | XbaI | NbHsc70-2-KpnI R | ATATTAATGTCGACGGTACCATCAACTTCCTCAATCTTAGGACC | KpnI | RNAi NbHsc70-2 F | TAAGGTTACCGAATTCATTCTTTCAGGTGAGGGTAATGAG | EcoRI | RNAi NbHsc70-2 R | AGACGCGTGAGCTCGGTACCCAGGAGGAATACCAGAAAGTTC | KpnI | NbHsc70-2-AhdI F | AGCAGGCTTTGACTTTAGGTCATGCGTATTATTAACGAGCCTACTG | AhdI | NbHsc70-2-AhdI R | TGGGTCTAGAGACTTTAGGTCTTAATCAACTTCCTCAATCTTAGGA | AhdI | NbHsc70-2qRT-F | AGGCTCCACTAGGATTCCGAAGG | | NbHsc70-2qRT-R | AGTGACATCCAACAGCAACAGGTC | | PVY-CP-F | GATGAATGGGCTTATGGTTTGGTG | | PVY-CP-R | GATTTGCCTAAGGGTTGGTTTCG | | Actin-F | CAAGGAAATCACCGCTTTGG | | Actin-R | AAGGGATGCGAGGATGGA |
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