HIGS-SsCCS转基因拟南芥的菌核病抗性鉴定
柴亚茹,丁一娟,周思钰,杨文静,闫宝琴,远俊虎,钱伟(
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Identification of the Resistance to Sclerotinia Stem Rot in HIGS-SsCCS Transgenic Arabidopsis thaliana
YaRu CHAI,YiJuan DING,SiYu ZHOU,WenJing YANG,BaoQin YAN,JunHu YUAN,Wei QIAN()
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图2. SsCCS HIGS载体的构建
A：SsCCS沉默表达载体构建模式图The pattern of vector construction to silence SsCCS;B：SsCCS特异干扰片段扩增Amplification of specific interference fragment of SsCCS;C：p-ccs载体转化大肠杆菌DH5α菌液检测结果Colony PCR verification of Escherichia coli DH5α transformed with p-ccs vector;D：EcoRI和XhoI双酶切鉴定R-ccs Identification of R-ccs vector by double digestion with EcoRI/XhoI enzymes;E：R-ccs载体转化农杆菌GV3101的菌落PCR鉴定Colony PCR identification of Agrobacterium tumefaciens GV3101 transformed with R-ccs vector;M：D2000 Marker
Fig. 2. Construction of HIGS vector of SsCCS
