HIGS-SsCCS转基因拟南芥的菌核病抗性鉴定
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柴亚茹,丁一娟,周思钰,杨文静,闫宝琴,远俊虎,钱伟
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Identification of the Resistance to Sclerotinia Stem Rot in HIGS-SsCCS Transgenic Arabidopsis thaliana
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YaRu CHAI,YiJuan DING,SiYu ZHOU,WenJing YANG,BaoQin YAN,JunHu YUAN,Wei QIAN
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表2 转基因拟南芥分离比及接种核盘菌后病斑统计
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Table 2 Segregation and lesion analyses of transgenic and wild type A. thaliana lines after inoculated with S. sclerotiorum
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编号
Code | T1代 T1 generation | T2代病斑面积
T2 generation lesion area
(cm2) | 阳性种子数
Positive | 非阳性种子数
Negative | 分离比
Segregation ratio | 病斑面积
Lesion area (cm2) | | WT | - | - | - | 1.46±0.26a | 1.02±0.36a | | HIGS-CCS-13 | 73 | 27 | 3﹕1 | 0.69±0.2b | 0.79±0.27b | | HIGS-CCS-8 | 69 | 31 | 3﹕1 | 0.71±0.17b | 0.48±0.21b | | HIGS-CCS-5 | 78 | 22 | 3﹕1 | 0.89±0.13b | 0.55±0.23b | | HIGS-CCS-14 | 79 | 21 | 3﹕1 | 0.82±0.27b | 0.93±0.27a | | HIGS-CCS-7 | 92 | 8 | 15﹕1 | 0.77±0.18b | | | HIGS-CCS-18 | 69 | 31 | 3﹕1 | 0.91±0.53a | | | HIGS-CCS-22 | 85 | 25 | 3﹕1 | 1.11±0.08a | | | HIGS-CCS-3 | 71 | 29 | 3﹕1 | 1.11±0.25a | | | HIGS-CCS-19 | 44 | 56 | 1﹕1 | 1.12±0.11a | | | HIGS-CCS-4 | 56 | 44 | 1﹕1 | 1.15±0.13a | | | HIGS-CCS-17 | 100 | 0 | - | 1.27±0.28a | | | HIGS-CCS-9 | 78 | 22 | 3﹕1 | 1.37±0.18a | | | HIGS-CCS-11 | 95 | 5 | 15﹕1 | 1.21±0.24a | | | HIGS-CCS-2 | 70 | 30 | 3﹕1 | 0.91 | | | HIGS-CCS-20 | 76 | 34 | 3﹕1 | 1.02 | | | HIGS-CCS-1 | 89 | 11 | 15﹕1 | 1.13 | | | HIGS-CCS-12 | 74 | 26 | 3﹕1 | 1.26 | |
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