103份小麦品种（系）抗条锈性和遗传多样性评价及基因检测

徐默然,蔺瑞明,王凤涛,冯晶,徐世昌

Evaluation of Resistance to Stripe Rust and Genetic Diversity and Detection of Resistance Genes in 103 Wheat Cultivars (Lines)

MoRan XU,RuiMing LIN,FengTao WANG,Jing FENG,ShiChang XU

表1 用于检测小麦抗条锈病基因的分子标记

Table 1 Molecular markers for the detection of the known resistance genes to stripe rust

基因 Gene	引物名称 Primer name	引物序列 Primer sequence	大小 Size (bp)	参考文献 Reference
Yr5	Yr5_insertion_F	CTCACGCATTTGACCATATACAACT	+1281	文献[22] Reference[22]
Yr5	Yr5_insertion_R	TATTGCATAACATGGCCTCCAGT	+1281	文献[22] Reference[22]
Yr9	AF1/AF4_F	GGAGACATCATGAAACATTTG	+1500	文献[23] Reference[23]
	AF1/AF4_R	CTGTTGTTGGGCAGAAAG	+1500	文献[23] Reference[23]
	H20F	GTTGGAAGGGAGCTCGAGCTG	+1598	文献[24] Reference[24]
	H20R	GTTGGGCAGAAAGGTCGACATC	+1598	文献[24] Reference[24]
Yr10	Yr10F/Yr10R_F	TCAAAGACATCAAGAGCCGC	+543	文献[25] Reference[25]
	Yr10F/Yr10R_R	TGGCCTACATGAACTCTGGAT	+543
	Yr10F1/Yr10R1_F	TTGGAATTGGCGACAAGCGT	+755
	Yr10F1/Yr10R1_R	GTGATGATTACCCACTTCCTC	+755
Yr15	Yr15K1-F	GGAGATAGAGCACATTACAGAC	+992	文献[26] Reference[26]
Yr15	Yr15K1-R	TTTCGCATCCCACCCTACTG	+992	文献[26] Reference[26]
Yr18	csLV34_F	TTGGTTAAGACTGGTGATGG	+150	文献[27] Reference[27]
	csLV34_R	TGCTTGCTATTGCTGAATAGT	-229	文献[27] Reference[27]
	L34SPF	GGGAGCA TT A TTTTTTTCCA TCA TG	+751	文献[28] Reference[28]
	L34DINT13R2	ACTTTCCTGAAAATAATACAAGCA	+751
	L34DINT9F	TTGATGAAACCAGTTTTTTTTCTA	-523
	L34MINUSR	TATGCCATTTAACATAATCATGAA	-523
Yr26	WE173_F	GGGACAAGGGGAGTTGAAGC	+451 -730	文献[29] Reference[29]
Yr26	WE173_R	GAGAGTTCCAAGCAGAACAC	+451 -730	文献[29] Reference[29]