基于RNA-seq数据的栽培种花生SSR位点鉴定和标记开发
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徐志军,赵胜,徐磊,胡小文,安东升,刘洋
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Discovery of Microsatellite Markers from RNA-seq Data in Cultivated Peanut (Arachis hypogaea)
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ZhiJun XU,Sheng ZHAO,Lei XU,XiaoWen HU,DongSheng AN,Yang LIU
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表4 基因关联SSR引物e-PCR扩增位点统计
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Table 4 Statistics of gene-associated SSR primer amplified in peanut genome by e-PCR
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DNA模板
DNA template | 扩增位点
Amplified loci | 有效扩增位点
Effective amplified loci | 引物扩增位点统计 Primer amplified loci statistics | 有效引物数
Number of effective primer pairs | | 1 | 2 | 3 | >3 | | A.duranensis | 4760 | 4468
(93.86%) | 3721
(93.75%) | 152
(3.83%) | 47
(1.18%) | 49
(1.23%) | 3969
(67.74%) | | A.ipaensis | 5264 | 4929
(93.64%) | 3866
(91.33%) | 249
(5.88%) | 52
(1.23%) | 66
(1.56%) | 4233
(72.25%) | | A.hypogaea | 10818 | 10188
(94.17%) | 1477
(28.54%) | 3221
(62.24%) | 252
(4.87%) | 225
(4.35%) | 5175
(88.33%) |
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