基于RNA-seq数据的栽培种花生SSR位点鉴定和标记开发

徐志军,赵胜,徐磊,胡小文,安东升,刘洋

Discovery of Microsatellite Markers from RNA-seq Data in Cultivated Peanut (Arachis hypogaea)

ZhiJun XU,Sheng ZHAO,Lei XU,XiaoWen HU,DongSheng AN,Yang LIU

表2 栽培种花生SSR各重复单元重复次数及分布频率

Table 2 Repetition times and distribution frequency of each SSR repeat unit in cultivated peanut

重复单元类型 Repeat unit type 重复次数Repetition times 5 6 7 8 9 10 11 12 13 14 ≥15 单核苷酸Mononucleotide 2985 1519 943 657 468 941 二核苷酸 Dinucleotide 1446 896 640 475 340 115 14 三核苷酸 Trinucleotide 4352 2108 761 130 四核苷酸 Tetranucleotide 246 59 五核苷酸 Pentanucleotide 48 总计 Total 4646 3613 1657 770 475 3325 1634 957 657 468 941 分布频率 Distribution frequency(%) 24.2 18.87 8.66 4.02 2.48 17.37 8.54 5.00 3.43 2.44 4.92