多杀性巴氏杆菌荚膜的生物合成及其调控机制研究进展

关丽君,薛云,丁文文,赵战勤

Advances in Mechanisms of Biosynthesis and Regulation of Pasteurella multocida Capsule

LiJun GUAN,Yun XUE,WenWen DING,ZhanQin ZHAO

表1 I类荚膜生物合成基因簇及编码蛋白的功能

Table 1 Biosynthesis gene clusters and protein-coding function of class I capsule

功能区 Region 开放阅读框（ORF） A型/D型/F型编码的蛋白质 Protein encoded by type A/D/F 功能 Function A型 Type A D型 Type B F型 Type D R1 hexA hexA hexA HexA ATP结合蛋白 ATP-binding protein hexB hexB hexB HexB 内膜蛋白 Inner membrane protein hexC hexC hexC HexC 具有周质结构域的内膜蛋白 Inner membrane protein with a periplasmic domain hexD hexD hexD HexD 外膜蛋白（脂蛋白） Outer membrane protein (lipoprotein) R2 hyaB dcbB fcbB HyaB/DcbB/FcbB 糖基转移酶 Glycosyltransferase hyaC dcbC fcbC HyaC/DcbC/FcbC UDP-葡萄糖脱氢酶,催化UDP-葡萄糖转化为UDP-葡糖醛酸 UDP-glucose dehydrogenase, catalyzing the conversion of UDP-glucose to UDP-glucuronic acid hyaD dcbF fcbD HyaD/DcbF/FcbD 透明质酸合成酶/肝素合成酶/软骨素合成酶 PmHAS/PmHS/PmCS hyaE dcbE fcbE HyaE/ DcbE / FcbE 起始酶,将重复单元的第一个残基转移至 Poly-KDO linker Initiates enzyme, which transfers from the first residue of the repeat-unit to the poly-KDO linker R3 phyA phyA phyA PhyA β-KDO糖基转移酶 β-Kdo glycosyltransferase phyB phyB phyB PhyB β-KDO糖基转移酶 β-Kdo glycosyltransferase