基于全转录组测序的绵羊胚胎不同发育阶段 骨骼肌circRNA的分析与鉴定
石田培,王欣悦,侯浩宾,赵志达,尚明玉,张莉(
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Analysis and Identification of circRNAs of Skeletal Muscle at Different Stages of Sheep Embryos Based on Whole Transcriptome Sequencing
SHI TianPei,WANG XinYue,HOU HaoBin,ZHAO ZhiDa,SHANG MingYu,ZHANG Li()
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图9. 实时荧光定量检测
图中为RNA-seq结果和qRT-PCR的定量结果;折线图表示测序的FPKM值,柱状图表示相对定量结果;显著性检验：D105柱状图上标符号为D85 vs D105比较结果;D135柱状图上2个标符号,左边为D85 vs D135比较结果,右边为D105 vs D135比较结果;* P≤0.05,** P≤0.01
Fig. 9. The results of qRT-PCR
The figure shows the quantitative results of RNA-seq and qRT-PCR; the line graph shows the FPKM value of the sequencing and the histogram shows the relative quantitative results; the significance test: the D105 histogram is marked with the D85 vs D105 comparison result; 2 test result in D135 histogram: left is D85 vs D135 and right is D105 vs D135; *P≤0.05, ** P≤0.01
