基于全转录组测序的绵羊胚胎不同发育阶段 骨骼肌circRNA的分析与鉴定

石田培,王欣悦,侯浩宾,赵志达,尚明玉,张莉

Analysis and Identification of circRNAs of Skeletal Muscle at Different Stages of Sheep Embryos Based on Whole Transcriptome Sequencing

TianPei SHI,XinYue WANG,HaoBin HOU,ZhiDa ZHAO,MingYu SHANG,Li ZHANG

表1 荧光定量引物基因列表

Table 1 The list of gene primers of qRT-PCR

RNA 类型 RNA Type RNA 名称 RNA ID 上游引物序列 Upstream primer sequence 下游引物序列 Downstream primer sequence circRNA内参基因 CircRNA reference gene Actin-β CGTGCGTGACATCAAAGAGAA AACCGCTCGTTGCCAATAGT circRNA circRNA17939 CTGGATACTGTGTTACTTAGAC CATTCTCAGAGGATTAACGATT circRNA circRNA1037 GGATGACTTGCCCAAGGAGAA CTTGATGGCGACTCGGACTT circRNA circRNA1042 GGAGATTGGTTTGGAGAGAAAGC TCCTGTCCTCATCATTATCGTCTG circRNA circRNA5212 TGCCCTCCTACAGACTTGA CGCTGGGTTGGAGATGTT miRNA内参基因 miRNA reference gene U6 CTCGCTTCGGCAGCACA AACGCTTCACGAATTTGCGT miRNA oar-miR-150 ACGCGTCTCCCAACCCTT GTCGTATCCAGTGCAGGGTCCGAGGTATTC GCACTGGATACGACCACTGGG miRNA oar-miR-410-5p CGGCGGAGGTTGTCTGTG GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCGAACT miRNA oar-miR-133a-5p GGCGGAGCTGGTAAAATGG GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACATTTGG miRNA bta-miR-365-3p CGCGTAATGCCCCTAAAAAT GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACATAAGG 通用下游 Universal downstream primer CAGTGCAGGGTCCGAGGTAT