超高效液相色谱-串联质谱法鉴定和分析稻米中 酚酸类化合物的组成及分布

赵珊,仲伶俐,周虹,李曦,雷欣宇,黄世群,郑幸果,冯俊彦,雷绍荣,郭灵安

Identification and Analysis of Phenolic Acids in Rice Using Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry

Shan ZHAO,LingLi ZHONG,Hong ZHOU,Xi LI,XinYu LEI,ShiQun HUANG,XingGuo ZHENG,JunYan FENG,ShaoRong LEI,LingAn GUO

表5 19种酚酸的线性方程、相关系数、检出限和定量限

Table 5 Linear equations, correlation coefficients, limits of detection (LODs) and limits of quantification (LOQs) of 19 kinds of phenolic acids

酚酸 Phenolic acid 线性范围 Linear range (μg·L-1) 回归方程 Linear equation 相关系数（R2） Correlation coefficient 检出限 LOD (μg·L-1) 定量限 LOQ (μg·L-1) 没食子酸Gallic acid 1.0—500 y=4129.01x+5097.625 0.99994 0.121 0.403 2,3,4-三羟基苯甲酸2,3,4-Trihydroxybenzoic acid 1.0—500 y=1181.706x+1184.094 0.99998 0.178 0.592 3,5-二羟基苯甲酸3,5-Dihydroxybenzoic acid 1.0—500 y=4200.251x+6932.746 0.99981 0.577 1.922 原儿茶酸Protocatechuic acid 1.0—500 y=5195.678x+4966.813 0.99998 0.221 0.735 龙胆酸Gentisic acid 5.0—500 y=1143.852x-5212.479 0.99974 4.728 15.759 焦儿茶酸Pyrocatechuic acid 2.5—500 y=1578.332 x-2815.838 0.99976 1.391 4.637 对羟基苯甲酸p-Hydroxybenzoic acid 1.0—500 y=8060.058x+89591.57 0.99999 0.023 0.076 绿原酸Chlorogenic Acid 1.0—500 y=3455.433x+915.729 0.99999 0.029 0.097 香草酸Vanillic acid 1.0—250 y=791.212x+5290.2 0.99993 0.060 0.201 咖啡酸Caffeic acid 2.5—250 y=9980.757x+29851.52 0.99962 1.799 5.995 丁香酸Syringic acid 1.0—500 y=1194.07x-734.952 0.99989 0.047 0.158 4-香豆酸p-Coumaric acid 1.0—500 y=11839.49x+4169.524 0.99999 0.144 0.479 水杨酸Salicylic acid 1.0—500 y=12668.42x-8192.132 0.99989 0.126 0.420 阿魏酸Ferulic acid 1.0—500 y=3638.813x-2875.187 0.99998 0.110 0.368 3-羟基肉桂酸3-Hydroxycinnamic acid 1.0—500 y=9461.071x+10025.18 0.99998 0.122 0.408 芥子酸Sinapic acid 1.0—500 y=2006.256x-2051.911 0.99998 0.050 0.167 异阿魏酸Isoferulic acid 1.0—500 y=929.839x-1868.854 0.99966 0.227 0.756 2-羟基肉桂酸2-Hydroxycinnamic acid 1.0—500 y=2820.271x+375.238 0.99999 0.457 1.522 反式肉桂酸trans-Cinnamic acid 1.0—250 y=3065.769x+3429.845 0.99973 0.293 0.976