超高效液相色谱-串联质谱法鉴定和分析稻米中 酚酸类化合物的组成及分布
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赵珊,仲伶俐,周虹,李曦,雷欣宇,黄世群,郑幸果,冯俊彦,雷绍荣,郭灵安
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Identification and Analysis of Phenolic Acids in Rice Using Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry
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Shan ZHAO,LingLi ZHONG,Hong ZHOU,Xi LI,XinYu LEI,ShiQun HUANG,XingGuo ZHENG,JunYan FENG,ShaoRong LEI,LingAn GUO
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表4 稻米样品在不同试验方法中添加酚酸的回收率结果（n=6）
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Table 4 Recoveries of phenolic acids in rice samples under different experimental condition (n=6)
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酚酸
Phenolic acid | 游离型提取Free type extraction (%) | 碱水解Alkaline hydrolysis (%) | 酸水解
Acid hydrolysis (%) | RSD
(%) | HLB固相萃取法
HLB Solid-phase extraction | RSD
(%) | 乙酸乙酯萃取法
Ethyl acetate extraction | RSD
(%) | NaOH | RSD
(%) | NaOH+1%抗坏血酸 NaOH+1% ascorbic acid | RSD
(%) | NaOH +1%抗坏血酸+10 mmol∙L-1 EDTA
NaOH +1% ascorbic acid+10 mmol∙L-1 EDTA | RSD
(%) | | 没食子酸Gallic acid | 96.3 | 2.95 | 75.7 | 0.83 | 0.0 | - | 87.4 | 4.79 | 92.1 | 2.11 | 73.5 | 2.47 | 2,3,4-三羟基苯甲酸
2,3,4-Trihydroxybenzoic acid | 92.6 | 1.93 | 95.4 | 2.02 | 0.0 | - | 86.9 | 3.41 | 94.5 | 1.80 | 86.9 | 0.66 | 3,5-二羟基苯甲酸
3,5-Dihydroxybenzoic acid | 102.1 | 0.20 | 86.7 | 4.65 | 95.2 | 2.81 | 97.2 | 1.65 | 97.5 | 0.79 | 96.5 | 0.61 | | 原儿茶酸Protocatechuic acid | 100.3 | 1.39 | 88.2 | 0.01 | 0.0 | - | 95.1 | 0.98 | 98.8 | 1.99 | 92.1 | 2.05 | | 龙胆酸Gentisic acid | 87.7 | 3.80 | 84.1 | 2.89 | 0.0 | - | 85.5 | 4.63 | 96.0 | 4.20 | 100.3 | 0.43 | | 焦儿茶酸Pyrocatechuic acid | 93.1 | 2.20 | 84.3 | 2.81 | 0.0 | - | 91.9 | 5.88 | 103.0 | 0.24 | 99.8 | 0.81 | 对羟基苯甲酸
p-Hydroxybenzoic acid | 99.0 | 0.47 | 95.0 | 0.55 | 99.9 | 0.12 | 99.5 | 2.36 | 103.1 | 2.03 | 96.2 | 1.86 | | 绿原酸Chlorogenic Acid | 89.9 | 1.01 | 55.3 | 3.98 | - | - | - | - | - | - | - | - | | 香草酸Vanillic acid | 103.8 | 1.90 | 92.3 | 1.34 | 101.8 | 0.19 | 98.6 | 1.28 | 100.5 | 1.74 | 92.1 | 3.87 | | 咖啡酸Caffeic acid | 90.2 | 0.59 | 93.1 | 2.53 | 0.0 | - | 92.9 | 1.50 | 100.1 | 1.64 | 64.1 | 1.63 | | 丁香酸Syringic acid | 102.9 | 1.28 | 92.6 | 2.19 | 94.1 | 1.40 | 98.8 | 0.42 | 100.9 | 2.30 | 95.9 | 1.00 | | 4-香豆酸p-Coumaric acid | 93.1 | 2.82 | 94.8 | 1.40 | 90.2 | 0.67 | 104.8 | 2.94 | 100.8 | 2.01 | 51.7 | 3.77 | | 水杨酸Salicylic acid | 89.1 | 2.39 | 85.6 | 0.72 | 90.5 | 0.09 | 98.4 | 1.68 | 100.6 | 1.14 | 78.5 | 1.73 | | 阿魏酸Ferulic acid | 97.5 | 3.00 | 98.0 | 1.05 | 84.4 | 1.61 | 110.5 | 0.12 | 103.1 | 0.28 | 52.3 | 3.55 | 3-羟基肉桂酸
3-Hydroxycinnamic acid | 98.1 | 0.95 | 91.8 | 2.49 | 97.8 | 1.18 | 100.0 | 3.15 | 100.9 | 2.42 | 92.5 | 1.64 | | 芥子酸Sinapic acid | 85.3 | 4.03 | 88.3 | 4.67 | 6.9 | 34.0 | 101.4 | 1.11 | 101.9 | 3.35 | 62.2 | 3.03 | | 异阿魏酸Isoferulic acid | 111.4 | 3.90 | 91.3 | 0.01 | 100.8 | 0.69 | 97.8 | 3.09 | 100.9 | 1.66 | 70.6 | 1.84 | 2-羟基肉桂酸
2-Hydroxycinnamic acid | 94.4 | 2.88 | 89.9 | 0.01 | 94.4 | 0.70 | 101.1 | 4.28 | 102.1 | 1.60 | 86.9 | 1.64 | 反式肉桂酸
trans-Cinnamic acid | 97.0 | 4.08 | 74.7 | 2.57 | 82.9 | 1.43 | 101.1 | 3.36 | 104.8 | 1.95 | 68.2 | 3.58 |
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