超高效液相色谱-串联质谱法鉴定和分析稻米中 酚酸类化合物的组成及分布

赵珊,仲伶俐,周虹,李曦,雷欣宇,黄世群,郑幸果,冯俊彦,雷绍荣,郭灵安

Identification and Analysis of Phenolic Acids in Rice Using Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry

Shan ZHAO,LingLi ZHONG,Hong ZHOU,Xi LI,XinYu LEI,ShiQun HUANG,XingGuo ZHENG,JunYan FENG,ShaoRong LEI,LingAn GUO

表3 目标化合物的质谱分析参数

Table 3 UPLC-MS/MS parameters for target compounds

酚酸 Phenolic acid 保留时间 Retention time (min) 质荷比Mass-to-charge ratio (m/z) 锥孔电压 Cone voltage (V) 碰撞能量Collision energy (eV) 离子扫描模式 Ion scanning mode 母离子 Parent ion 子离子 Daughter ion 没食子酸Gallic acid 1.89 168.90 124.91*、78.90 28 14,22 负离子 Negative ions 2,3,4-三羟基苯甲酸 2,3,4-Trihydroxybenzoic acid 2.87 168.90 150.94*、106.89 32 14,18 负离子Negative ions 3,5-二羟基苯甲酸 3,5-Dihydroxybenzoic acid 2.97 152.90 108.90*、64.97 14 12,12 负离子Negative ions 原儿茶酸Protocatechuic acid 3.24 152.90 108.90*、90.88 6 14,22 负离子Negative ions 龙胆酸Gentisic acid 4.64 152.90 108.90*、80.92 10 12,16 负离子Negative ions 焦儿茶酸Pyrocatechuic acid 4.65 152.90 108.90*、80.92 14 16,22 负离子Negative ions 对羟基苯甲酸 p-Hydroxybenzoic acid 4.69 136.90 92.89*、64.91 6 12,24 负离子Negative ions 绿原酸Chlorogenic acid 4.85 353.03 190.99*、84.88 18 20,40 负离子Negative ions 香草酸Vanillic acid 5.85 166.90 151.93*、107.92 8 12,22 负离子Negative ions 咖啡酸Caffeic acid 5.98 178.90 134.93*、106.88 27 16,24 负离子Negative ions 丁香酸Syringic acid 6.61 196.97 181.96*、122.89 18 14,24 负离子Negative ions 4-香豆酸p-Coumaric acid 8.97 162.97 118.92*、93.15 22 12,20 负离子Negative ions 水杨酸Salicylic acid 10.25 136.90 92.89*、64.91 16 14,22 负离子Negative ions 阿魏酸Ferulic acid 11.06 192.97 133.95*、177.95 14 18,12 负离子Negative ions 3-羟基肉桂酸 3-Hydroxycinnamic acid 11.49 162.97 118.92*、90.93 22 16,24 负离子Negative ions 芥子酸Sinapic acid 11.65 223.03 207.97*、163.93 16 14,16 负离子Negative ions 异阿魏酸Isoferulic acid 12.09 192.97 177.95*、133.96 6 12,14 负离子Negative ions 2-羟基肉桂酸 2-Hydroxycinnamic acid 13.64 162.97 119.18*、92.89 2 24,24 负离子Negative ions 反式肉桂酸 trans-Cinnamic acid 14.50 148.90 130.92*、102.94 38 10,18 正离子Positive ions