玉米大斑病菌和小斑病菌交配型多重PCR 检测方法的建立与应用

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代玉立¹,甘林¹,滕振勇²,杨静民³,祁月月⁴,石妞妞¹,陈福如¹,杨秀娟¹(

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Establishment and Application of a Multiple PCR Method to Detect Mating Types of Exserohilum turcicum and Bipolaris maydis

DAI YuLi¹,GAN Lin¹,TENG ZhenYong²,YANG JingMin³,QI YueYue⁴,SHI NiuNiu¹,CHEN FuRu¹,YANG XiuJuan¹(

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图4. 玉米大斑病菌和小斑病菌交配型多重PCR检测的特异性
M：2 kb DNA ladder;1—6：分别为3株MAT1-1和MAT1-2的玉米大斑病菌（A）和小斑病菌（B）菌株Three MAT1-1 and MAT1-2 strains of E. turcicum (A) and B. maydis (B), respectively;7—10：表1所示的4株玉米大斑病菌（A）和小斑病菌（B）近缘种菌株Four strains of closely related species of Exserohilum (A) and Bipolaris (B), respectively, listed in Table 1;11—24：表1所示的14株其他真菌菌株Fourteen strains of other fungal species listed in Table 1

Fig. 4. Specificity of the multiple PCR to detect mating types of E. turcicum and B. maydis