玉米大斑病菌和小斑病菌交配型多重PCR 检测方法的建立与应用
代玉立1,甘林1,滕振勇2,杨静民3,祁月月4,石妞妞1,陈福如1,杨秀娟1(
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Establishment and Application of a Multiple PCR Method to Detect Mating Types of Exserohilum turcicum and Bipolaris maydis
DAI YuLi1,GAN Lin1,TENG ZhenYong2,YANG JingMin3,QI YueYue4,SHI NiuNiu1,CHEN FuRu1,YANG XiuJuan1()
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图3. 玉米大斑病菌和小斑病菌交配型多重PCR检测的灵敏度
M：2 kb DNA ladder;1—7：（A）玉米大斑病菌MAT1-1和MAT1-2交配型菌株的基因组DNA的量为100、10、1、0.1、0.01、0.001和0.0001 ng Genomic DNA from MAT1-1 and MAT1-2 strains of E. turcicum at amounts of 100, 10, 1, 0.1, 0.01, 0.001 and 0.0001 ng, respectively;（B）玉米小斑病菌MAT1-1和MAT1-2交配型菌株的基因组DNA的量为10、1、0.1、0.01、0.001、0.0001和0.00001 ng Genomic DNA from MAT1-1 and MAT1-2 strains of B. maydis at amounts of 10, 1, 0.1, 0.01, 0.001, 0.0001 and 0.00001 ng, respectively;8：空白对照Reagent only without DNA template
Fig. 3. Sensitivity test of the multiple PCR to detect mating types of E. turcicum and B. maydis
