玉米大斑病菌和小斑病菌交配型多重PCR 检测方法的建立与应用

代玉立,甘林,滕振勇,杨静民,祁月月,石妞妞,陈福如,杨秀娟

Establishment and Application of a Multiple PCR Method to Detect Mating Types of Exserohilum turcicum and Bipolaris maydis

YuLi DAI,Lin GAN,ZhenYong TENG,JingMin YANG,YueYue QI,NiuNiu SHI,FuRu CHEN,XiuJuan YANG

表3 不同地理来源的玉米大斑病菌和小斑病菌交配型的多重PCR和杂交检测

Table 3 Multiple PCR and hybridization detection of mating types of E. turcicum and B. maydis strains from different geographic origins

菌株来源 Source of strains	病原菌 Pathogen	菌株数量 Strain numbers ^a	交配型的多重PCR和杂交检测结果 Results of mating type detection using multiple PCR and cross assay ^b
菌株来源 Source of strains	病原菌 Pathogen	菌株数量 Strain numbers ^a	MAT1-1	MAT1-2
福建建瓯Jianou, Fujian	玉米大斑病菌E. turcicum	64 (10)	+ (2)	+ (8)
福建建瓯Jianou, Fujian	玉米小斑病菌B. maydis	12 (5)	+	+ (5)
福建松溪Songxi, Fujian	玉米大斑病菌E. turcicum	20 (8)	+ (1)	+ (7)
福建屏南Pingnan, Fujian	玉米大斑病菌E. turcicum	15 (5)	+	+ (5)
福建屏南Pingnan, Fujian	玉米小斑病菌B. maydis	24 (10)	+ (3)	+ (7)
福建福州Fuzhou, Fujian	玉米小斑病菌B. maydis	20 (8)	+ (2)	+ (6)
福建莆田Putian, Fujian	玉米大斑病菌E. turcicum	1	ND	+
福建莆田Putian, Fujian	玉米小斑病菌B. maydis	8 (5)	+	+ (5)
福建南靖Nanjing, Fujian	玉米大斑病菌E. turcicum	20 (10)	+ (1)	+ (9)
福建南靖Nanjing, Fujian	玉米小斑病菌B. maydis	11 (5)	+	+ (5)
福建龙岩Longyan, Fujian	玉米大斑病菌E. turcicum	8 (5)	+	+ (5)
福建龙岩Longyan, Fujian	玉米小斑病菌B. maydis	9 (5)	+ (1)	+ (4)
安徽霍邱Huoqiu, Anhui	玉米小斑病菌B. maydis	26 (8)	+ (1)	+ (7)
海南三亚Sanya, Hainan	玉米大斑病菌E. turcicum	1	ND	+
海南三亚Sanya, Hainan	玉米小斑病菌B. maydis	24 (6)	+ (2)	+ (4)
浙江泰顺Taishun, Zhejiang	玉米小斑病菌B. maydis	27 (8)	+ (3)	+ (5)
广东Guangdong	玉米小斑病菌B. maydis	33 (10)	+ (4)	+ (6)
总计 Total	玉米大斑病菌E. turcicum	129 (38)	+ (4)	+ (34)
总计 Total	玉米小斑病菌B. maydis	194 (70)	+ (16)	+ (54)